1drz: Difference between revisions
New page: left|200px<br /> <applet load="1drz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1drz, resolution 2.3Å" /> '''U1A SPLICEOSOMAL PRO... |
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'''U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX'''<br /> | '''U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1DRZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1DRZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DRZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: u1a]] | [[Category: u1a]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:40:41 2008'' | ||
Revision as of 13:40, 15 February 2008
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U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX
Overview
The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only, catalytic RNA known to be required for the viability of a human pathogen., We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic, HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the, RNA to bind a small, basic protein without affecting ribozyme activity., The co-crystal structure shows that the compact catalytic core comprises, five helical segments connected as an intricate nested double pseudoknot., The 5'-hydroxyl leaving group resulting from the self-scission reaction is, buried deep within an active-site cleft produced by juxtaposition of the, helices and five strand-crossovers, and is surrounded by biochemically, important backbone and base functional groups in a manner reminiscent of, protein enzymes.
About this Structure
1DRZ is a Single protein structure of sequence from Hepatitis delta virus and Homo sapiens with MG and SO4 as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582
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