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==C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II==
==C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II==
<StructureSection load='1rmj' size='340' side='right'caption='[[1rmj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1rmj' size='340' side='right'caption='[[1rmj]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1rmj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RMJ FirstGlance]. <br>
<table><tr><td colspan='2'>[[1rmj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RMJ FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IGFBP6, IBP6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rmj OCA], [https://pdbe.org/1rmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rmj RCSB], [https://www.ebi.ac.uk/pdbsum/1rmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rmj ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rmj OCA], [https://pdbe.org/1rmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rmj RCSB], [https://www.ebi.ac.uk/pdbsum/1rmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rmj ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/IBP6_HUMAN IBP6_HUMAN]] IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.  
[https://www.uniprot.org/uniprot/IBP6_HUMAN IBP6_HUMAN] IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rmj ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rmj ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
IGFs are important mediators of growth. IGF binding proteins (IGFBPs) 1-6 regulate IGF actions and have IGF-independent actions. The C-terminal domains of IGFBPs contribute to high-affinity IGF binding and modulation of IGF actions and confer some IGF-independent properties, but understanding how they achieve this has been constrained by the lack of a three-dimensional structure. We therefore determined the solution structure of the C-domain of IGFBP-6 using nuclear magnetic resonance (NMR). The domain consists of a thyroglobulin type 1 fold comprising an alpha-helix followed by a loop, a three-stranded antiparallel beta-sheet incorporating a second loop, and finally a disulfide-bonded flexible third loop. The IGF-II binding site on the C-domain was identified by examining NMR spectral changes upon complex formation. It consists of a largely hydrophobic surface patch involving the alpha-helix, the first beta-strand, and the first and second loops. The site was confirmed by mutagenesis of several residues, which resulted in decreased IGF binding affinity. The IGF-II binding site lies adjacent to surfaces likely to be involved in glycosaminoglycan binding of IGFBPs, which might explain their decreased IGF affinity when bound to glycosaminoglycans, and nuclear localization. Our structure provides a framework for understanding the roles of IGFBP C-domains in modulating IGF actions and conferring IGF-independent actions, as well as ultimately for the development of therapeutic IGF inhibitors for diseases including cancer.
C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II.,Headey SJ, Keizer DW, Yao S, Brasier G, Kantharidis P, Bach LA, Norton RS Mol Endocrinol. 2004 Nov;18(11):2740-50. Epub 2004 Aug 12. PMID:15308688<ref>PMID:15308688</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1rmj" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Insulin-like growth factor binding protein 3D structures|Insulin-like growth factor binding protein 3D structures]]
*[[Insulin-like growth factor binding protein 3D structures|Insulin-like growth factor binding protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bach, L A]]
[[Category: Bach LA]]
[[Category: Brasier, G]]
[[Category: Brasier G]]
[[Category: Headey, S J]]
[[Category: Headey SJ]]
[[Category: Kantharidis, P]]
[[Category: Kantharidis P]]
[[Category: Keizer, D W]]
[[Category: Keizer DW]]
[[Category: Norton, R S]]
[[Category: Norton RS]]
[[Category: Yao, S]]
[[Category: Yao S]]
[[Category: Hormone-growth factor complex]]
[[Category: Insulin-like growth factor binding protein]]

Revision as of 08:25, 1 May 2024

C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II

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