7ead: Difference between revisions
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==Crystal structure of beta-sheet cytochrome c prime from Thermus thermophilus.== | ==Crystal structure of beta-sheet cytochrome c prime from Thermus thermophilus.== | ||
<StructureSection load='7ead' size='340' side='right'caption='[[7ead]]' scene=''> | <StructureSection load='7ead' size='340' side='right'caption='[[7ead]], [[Resolution|resolution]] 1.74Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EAD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EAD FirstGlance]. <br> | <table><tr><td colspan='2'>[[7ead]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EAD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EAD FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ead FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ead OCA], [https://pdbe.org/7ead PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ead RCSB], [https://www.ebi.ac.uk/pdbsum/7ead PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ead ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ead FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ead OCA], [https://pdbe.org/7ead PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ead RCSB], [https://www.ebi.ac.uk/pdbsum/7ead PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ead ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cytochrome c'-beta is a heme protein that belongs to the cytochrome P460 family and consists of homodimeric subunits with a predominantly antiparallel beta-sheet fold. Here, the crystal structure of cytochrome c'-beta from the thermophilic Thermus thermophilus (TTCP-beta) is reported at 1.74 A resolution. TTCP-beta has a typical antiparallel beta-sheet fold similar to that of cytochrome c'-beta from the moderately thermophilic Methylococcus capsulatus (MCCP-beta). The phenylalanine cap structure around the distal side of the heme is also similar in TTCP-beta and MCCP-beta, indicating that both proteins similarly bind nitric oxide and carbon monoxide, as observed spectroscopically. Notably, TTCP-beta exhibits a denaturation temperature of 117 degrees C, which is higher than that of MCCP-beta. Mutational analysis reveals that the increased homodimeric interface area of TTCP-beta contributes to its high thermal stability. Furthermore, 14 proline residues, which are mostly located in the TTCP-beta loop regions, possibly contribute to the rigid loop structure compared with MCCP-beta, which has only six proline residues. These findings, together with those from phylogenetic analysis, suggest that the structures of Thermus cytochromes c'-beta, including TTCP-beta, are optimized for function under the high-temperature conditions in which the source organisms live. | |||
Crystal structure of thermally stable homodimeric cytochrome c'-beta from Thermus thermophilus.,Yoshimi T, Fujii S, Oki H, Igawa T, Adams HR, Ueda K, Kawahara K, Ohkubo T, Hough MA, Sambongi Y Acta Crystallogr F Struct Biol Commun. 2022 Jun 1;78(Pt 6):217-225. doi:, 10.1107/S2053230X22005088. Epub 2022 May 27. PMID:35647678<ref>PMID:35647678</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7ead" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Adams | [[Category: Adams, R H]] | ||
[[Category: Fujii S]] | [[Category: Fujii, S]] | ||
[[Category: Hough | [[Category: Hough, A M]] | ||
[[Category: Igawa T]] | [[Category: Igawa, T]] | ||
[[Category: Kawahara K]] | [[Category: Kawahara, K]] | ||
[[Category: Ohkubo T]] | [[Category: Ohkubo, T]] | ||
[[Category: Oki H]] | [[Category: Oki, H]] | ||
[[Category: Sambongi Y]] | [[Category: Sambongi, Y]] | ||
[[Category: Ueda K]] | [[Category: Ueda, K]] | ||
[[Category: Yoshimi T]] | [[Category: Yoshimi, T]] | ||
[[Category: Cytochrome c]] | |||
[[Category: Electron transport]] | |||
[[Category: Thermophile]] | |||
