Sandbox Reserved 1704: Difference between revisions
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=== Ligand Binding=== | === Ligand Binding=== | ||
The ligands utilized by anaplastic lymphoma kinase are monomeric FAM150 and dimeric AUG. It's biologically preferred ligand is dimeric AUG. | The ligands utilized by anaplastic lymphoma kinase are monomeric FAM150 and dimeric AUG. It's biologically preferred ligand is dimeric AUG. The binding of ALK to it's ligand causes results in homodimerization and a conformational change. Prior to the ligand binding to anaplastic lymphoma kinase, the extracellular domain is oriented sticking up perpendicularly to the plasma membrane. Once the ligand is bound, ALK undergoes a conformational change and folds over so that the positively charged residues on the portion of the protein previously sticking up perpendicularly is now interacting to the negatively charged residues on the plasma membrane. Once the ligand is bound and the conformational change has occurred, the kinase domain is auto-activated in which the domains use the tyrosine phosphorylation mechanism to phosphorylate the tyrosine residue on the opposite monomer. | ||
== Tyrosine Phosphorylation Mechanism == | ==== Tyrosine Phosphorylation Mechanism ==== | ||
Dimerization of anaplastic lymphoma kinase activates the kinase domains of each monomer. Next, the kinase domains phosphorylate the tyrosine residues of the opposite monomer using ATP. These phosphorylated tyrosine residues recruit signal proteins through phosphorylation. These signal proteins begin a signaling cascade through utilizing various signal pathways. These pathways signal for cell proliferation and survival (ex: begin transcription). | Dimerization of anaplastic lymphoma kinase activates the kinase domains of each monomer. Next, the kinase domains phosphorylate the tyrosine residues of the opposite monomer using ATP. These phosphorylated tyrosine residues recruit signal proteins through phosphorylation. These signal proteins begin a signaling cascade through utilizing various signal pathways. These pathways signal for cell proliferation and survival (ex: begin transcription). | ||
