Sandbox Reserved 1706: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 23: | Line 23: | ||
The rotation of the domains between the <scene name='90/904311/Open_conformation/2'>open conformation</scene> and <scene name='90/904311/Closed_conformation/3'>closed conformation</scene> of Neurofibromin are conducted by three helical linkers named L1, L2, and L3. The <scene name='90/904312/Linker_closed/1'>linkers in the closed conformation</scene> | The rotation of the domains between the <scene name='90/904311/Open_conformation/2'>open conformation</scene> and <scene name='90/904311/Closed_conformation/3'>closed conformation</scene> of Neurofibromin are conducted by three helical linkers named L1, L2, and L3. The <scene name='90/904312/Linker_closed/1'>linkers in the closed conformation</scene> | ||
<scene name='90/904312/Linker_closed/2'>(zoomed in)'</scene> and the <scene name='90/904312/Linkers_open/2'>linkers in the open conformation</scene> | <scene name='90/904312/Linker_closed/2'>(zoomed in)'</scene> and the <scene name='90/904312/Linkers_open/2'>linkers in the open conformation</scene> | ||
<scene name='90/904312/Zoomed_lo/1'>(zoomed in)'</scene> undergo rotations to relocate the GRD and Sec14-PH sites. Linker 1 (L1) consists of a loop connected by two helices from L1173-M1215 and is the main contributor in rotation of the GRD domain. The rotation of L1 to the <scene name='90/904312/Linkers_open/2'>open conformation</scene> causes N-HEAT ARM [https://en.wikipedia.org/wiki/Alpha_helix alpha helix] 48 and GRD helix 49 to extend out, aligning to form a hinge point at G1190. The GRD relocation is assisted by Sec14-PH relocation, which is initiated by Linker 3(L3) from Q1835 to G1852 where the proline rich section of the C-HEAT ARM changes conformation.L1 and L3 move closer to each other in the <scene name='90/904312/Linkers_open/2'>open conformation</scene>. Linker 2 (L2) consists of residues G1547-T1565 and begins at helix 63, the final helix of the GRD site, and connects into the short loop of [https://en.wikipedia.org/wiki/Alpha_helix alpha helix] 65 of the Sec14-PH domain. The combination of these three linkers are responsible for the conformational shift of the <scene name='90/904312/Linker_closed/1'> closed conformation</scene> | <scene name='90/904312/Zoomed_lo/1'>(zoomed in)'</scene> undergo rotations to relocate the GRD and Sec14-PH sites. Linker 1 (L1) consists of a loop connected by two helices from L1173-M1215 and is the main contributor in rotation of the GRD domain. The rotation of L1 to the <scene name='90/904312/Linkers_open/2'>open conformation</scene> causes N-HEAT ARM [https://en.wikipedia.org/wiki/Alpha_helix alpha helix] 48 and GRD helix 49 to extend out, aligning to form a hinge point at G1190. The GRD relocation is assisted by Sec14-PH relocation, which is initiated by Linker 3(L3) from Q1835 to G1852 where the proline rich section of the C-HEAT ARM changes conformation.L1 and L3 move closer to each other in the <scene name='90/904312/Linkers_open/2'>open conformation</scene> allowing for the GRD and Sec14-PH domain to initiate their significant rearrangement. Linker 2 (L2) consists of residues G1547-T1565 and begins at helix 63, the final helix of the GRD site, and connects into the short loop of [https://en.wikipedia.org/wiki/Alpha_helix alpha helix] 65 of the Sec14-PH domain and also assists in shifting the Sec14-PH away from the GRD site. The combination of these three linkers are responsible for the conformational shift of the <scene name='90/904312/Linker_closed/1'> closed conformation</scene> | ||
<scene name='90/904312/Linker_closed/2'>(zoomed in)'</scene> and <scene name='90/904312/Linkers_open/2'>open conformation</scene> | <scene name='90/904312/Linker_closed/2'>(zoomed in)'</scene> and <scene name='90/904312/Linkers_open/2'>open conformation</scene> | ||
<scene name='90/904312/Zoomed_lo/1'>(zoomed in)'</scene>. | <scene name='90/904312/Zoomed_lo/1'>(zoomed in)'</scene>. | ||
