1e7k: Difference between revisions

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[[Image:1e7k.gif|left|200px]]<br />

[[Image:1e7k.gif|left|200px]]<br /><applet load="1e7k" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1e7k" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1e7k, resolution 2.9Å" />

'''CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT'''<br />

==Overview==

We have determined the crystal structure of a spliceosomal RNP complex, comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5', stem-loop of U4 snRNA. The protein interacts almost exclusively with a, purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual, RNA fold characterized by two tandem sheared G-A base pairs, a high degree, of purine stacking, and the accommodation of a single RNA base, rotated, out of the RNA chain, in a pocket of the protein. Apart from yielding the, structure of an important entity in the pre-mRNA splicing apparatus, this, work also implies a model for the complex of the 15.5kD protein with box, C/D snoRNAs. It additionally suggests a general recognition principle in a, novel family of RNA binding proteins.

We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.

==About this Structure==

1E7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA].

1E7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA].

==Reference==

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[[Category: u4 snrna]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Mon Nov~~ 12 16:40:~~33 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:24:41 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1e7k.gif|left|200px]]<br />
+[[Image:1e7k.gif|left|200px]]<br /><applet load="1e7k" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1e7k" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1e7k, resolution 2.9&Aring;" />
 '''CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT'''<br />
 ==Overview==
-We have determined the crystal structure of a spliceosomal RNP complex, comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5', stem-loop of U4 snRNA. The protein interacts almost exclusively with a, purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual, RNA fold characterized by two tandem sheared G-A base pairs, a high degree, of purine stacking, and the accommodation of a single RNA base, rotated, out of the RNA chain, in a pocket of the protein. Apart from yielding the, structure of an important entity in the pre-mRNA splicing apparatus, this, work also implies a model for the complex of the 15.5kD protein with box, C/D snoRNAs. It additionally suggests a general recognition principle in a, novel family of RNA binding proteins.
+We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.
 ==About this Structure==
-E7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA].
+E7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA].
 ==Reference==
@@ Line 23: / Line 22: @@
 [[Category: u4 snrna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:40:33 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:24:41 2008''