1gr0: Difference between revisions

Line 1:

[[Image:1gr0.~~gif~~|left|200px]]

[[Image:1gr0.png|left|200px]]

<!--

Line 9:

Line 10:

~~'''~~MYO-INOSITOL 1-PHOSPHATE SYNTHASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH NAD AND ZINC.~~'''~~

===MYO-INOSITOL 1-PHOSPHATE SYNTHASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH NAD AND ZINC.===

~~==Overview==~~

<!--

Phosphatidylinositol (PI) is essential for Mycobacterium tuberculosis viability and the enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents for which little structural information is available. The ~~rate-limiting step in the pathway is the production of (L)-myo-inositol 1-phosphate from (D)-glucose 6-phosphate~~, ~~a complex reaction catalyzed by~~ the ~~enzyme inositol 1-phosphate synthase. We have determined~~ the ~~crystal structure of this enzyme from Mycobacterium tuberculosis~~ (~~tbINO)~~ at 1.~~95 A resolution~~, ~~bound to the cofactor NAD+. The active site~~ is ~~located within a deep cleft at~~ the junction between two domains. The unexpected presence of a zinc ion here suggests a mechanistic difference from the eukaryotic inositol synthases, which are stimulated by monovalent cations, that may be exploitable in developing selective inhibitors of tbINO.

The line below this paragraph, {{ABSTRACT_PUBMED_12005437}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12005437 is the PubMed ID number.

-->

==About this Structure==

Line 32:

Line 36:

[[Category: Tb structural genomics consortium,tb]]

[[Category: Tbsgc]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ ~~2 17~~:54:44 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:55:49 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1gr0.gif|left|200px]]
+{{Seed}}
+[[Image:1gr0.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_1gr0|  PDB=1gr0  |  SCENE=  }}
-'''MYO-INOSITOL 1-PHOSPHATE SYNTHASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH NAD AND ZINC.'''
+===MYO-INOSITOL 1-PHOSPHATE SYNTHASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH NAD AND ZINC.===
-==Overview==
+<!--
-Phosphatidylinositol (PI) is essential for Mycobacterium tuberculosis viability and the enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents for which little structural information is available. The rate-limiting step in the pathway is the production of (L)-myo-inositol 1-phosphate from (D)-glucose 6-phosphate, a complex reaction catalyzed by the enzyme inositol 1-phosphate synthase. We have determined the crystal structure of this enzyme from Mycobacterium tuberculosis (tbINO) at 1.95 A resolution, bound to the cofactor NAD+. The active site is located within a deep cleft at the junction between two domains. The unexpected presence of a zinc ion here suggests a mechanistic difference from the eukaryotic inositol synthases, which are stimulated by monovalent cations, that may be exploitable in developing selective inhibitors of tbINO.
+The line below this paragraph, {{ABSTRACT_PUBMED_12005437}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12005437 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12005437}}
 ==About this Structure==
@@ Line 32: / Line 36: @@
 [[Category: Tb structural genomics consortium,tb]]
 [[Category: Tbsgc]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:54:44 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 05:55:49 2008''