5h8z

Crystal structure of the C49A C353A mutant Fenna-Matthews-Olson Protein from Chlorobaculum Tepidum

spinqualitylabelsall models 5h8z, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5h8z is a 2 chain structure with sequence from Chlorobaculum tepidum TLS. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: BCL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BCPA_CHLTE Intermediary in the transfer of excitation energy from the chlorophyll to the reaction centers. Publication Abstract from PubMed The Fenna-Matthews-Olson (FMO) pigment-protein complex in green sulfur bacteria transfers excitation energy from the chlorosome antenna complex to the reaction center. In understanding energy transfer in the FMO protein, the individual contributions of the bacteriochlorophyll pigments to the FMO complex's absorption spectrum could provide detailed information with which molecular and energetic models can be constructed. The absorption properties of the pigments, however, are such that their spectra overlap significantly. To overcome this, we used site-directed mutagenesis to construct a series of mutant FMO complexes in the model green sulfur bacterium Chlorobaculum tepidum (formerly Chlorobium tepidum). Two cysteines at positions 49 and 353 in the C. tepidum FMO complex, which reside near hydrogen bonds between BChls 2 and 3, and their amino acid binding partners serine 73 and tyrosine 15, respectively, were changed to alanine residues. The resulting C49A, C353A, and C49A C353A double mutants were analyzed with a combination of optical absorption and circular dichroism (CD) spectroscopies. Our results revealed changes in the absorption properties of several underlying spectral components in the FMO complex, as well as the redox behavior of the complex in response to the reductant sodium dithionite. A high-resolution X-ray structure of the C49A C353A double mutant reveals that these spectral changes appear to be independent of any major structural rearrangements in the FMO mutants. Our findings provide important tests for theoretical calculations of the C. tepidum FMO absorption spectrum, and additionally highlight a possible role for cysteine residues in the redox activity of the pigment-protein complex. Perturbation Of Bacteriochlorophyll Molecules In Fenna-Matthews-Olson Protein Complexes Through Mutagenesis Of Cysteine Residues.,Saer R, Orf GS, Lu X, Zhang H, Cuneo MJ, Myles DA, Blankenship RE Biochim Biophys Acta. 2016 Apr 22. pii: S0005-2728(16)30103-7. doi:, 10.1016/j.bbabio.2016.04.007. PMID:27114180 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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