7qbk: Difference between revisions
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==Crystal structure of a second homolog of R2-like ligand-binding oxidase in Sulfolobus acidocaldarius (SaR2loxII)== | ==Crystal structure of a second homolog of R2-like ligand-binding oxidase in Sulfolobus acidocaldarius (SaR2loxII)== | ||
<StructureSection load='7qbk' size='340' side='right'caption='[[7qbk]]' scene=''> | <StructureSection load='7qbk' size='340' side='right'caption='[[7qbk]], [[Resolution|resolution]] 2.26Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QBK FirstGlance]. <br> | <table><tr><td colspan='2'>[[7qbk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QBK FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qbk OCA], [https://pdbe.org/7qbk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qbk RCSB], [https://www.ebi.ac.uk/pdbsum/7qbk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qbk ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qbk OCA], [https://pdbe.org/7qbk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qbk RCSB], [https://www.ebi.ac.uk/pdbsum/7qbk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qbk ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
R2-like ligand-binding oxidase (R2lox) is a ferritin-like protein that harbours a heterodinuclear manganese-iron active site. Although R2lox function is yet to be established, the enzyme binds a fatty acid ligand coordinating the metal centre and catalyses the formation of a tyrosine-valine ether cross-link in the protein scaffold upon O2 activation. Here, we characterized the ligands copurified with R2lox by mass spectrometry-based metabolomics. Moreover, we present the crystal structures of two new homologs of R2lox, from Saccharopolyspora erythraea and Sulfolobus acidocaldarius, at 1.38 A and 2.26 A resolution, respectively, providing the highest resolution structure for R2lox, as well as new insights into putative mechanisms regulating the function of the enzyme. | |||
Comparative structural analysis provides new insights into the function of R2-like ligand-binding oxidase.,Diamanti R, Srinivas V, Johansson AI, Nordstrom A, Griese JJ, Lebrette H, Hogbom M FEBS Lett. 2022 Feb 17. doi: 10.1002/1873-3468.14319. PMID:35175627<ref>PMID:35175627</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7qbk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Diamanti R]] | [[Category: Diamanti, R]] | ||
[[Category: Hogbom M]] | [[Category: Hogbom, M]] | ||
[[Category: Lebrette H]] | [[Category: Lebrette, H]] | ||
[[Category: Srinivas V]] | [[Category: Srinivas, V]] | ||
[[Category: Ferritin-like superfamily]] | |||
[[Category: Metalloprotein]] | |||
[[Category: Mn/fe cofactor]] | |||
[[Category: Oxidoreductase]] | |||
[[Category: R2-like ligand-binding oxidase]] | |||
[[Category: R2lox]] | |||
[[Category: Ribonucleotide reductase r2 subunit fold]] | |||
