7qbp: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of R2-like ligand-binding oxidase from Saccharopolyspora Erythraea== | ==Crystal structure of R2-like ligand-binding oxidase from Saccharopolyspora Erythraea== | ||
<StructureSection load='7qbp' size='340' side='right'caption='[[7qbp]]' scene=''> | <StructureSection load='7qbp' size='340' side='right'caption='[[7qbp]], [[Resolution|resolution]] 1.38Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QBP FirstGlance]. <br> | <table><tr><td colspan='2'>[[7qbp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QBP FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qbp OCA], [https://pdbe.org/7qbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qbp RCSB], [https://www.ebi.ac.uk/pdbsum/7qbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qbp ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7qbk|7qbk]]</div></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qbp OCA], [https://pdbe.org/7qbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qbp RCSB], [https://www.ebi.ac.uk/pdbsum/7qbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qbp ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/RIR2H_SACEN RIR2H_SACEN]] Probable oxidase. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
R2-like ligand-binding oxidase (R2lox) is a ferritin-like protein that harbours a heterodinuclear manganese-iron active site. Although R2lox function is yet to be established, the enzyme binds a fatty acid ligand coordinating the metal centre and catalyses the formation of a tyrosine-valine ether cross-link in the protein scaffold upon O2 activation. Here, we characterized the ligands copurified with R2lox by mass spectrometry-based metabolomics. Moreover, we present the crystal structures of two new homologs of R2lox, from Saccharopolyspora erythraea and Sulfolobus acidocaldarius, at 1.38 A and 2.26 A resolution, respectively, providing the highest resolution structure for R2lox, as well as new insights into putative mechanisms regulating the function of the enzyme. | |||
Comparative structural analysis provides new insights into the function of R2-like ligand-binding oxidase.,Diamanti R, Srinivas V, Johansson AI, Nordstrom A, Griese JJ, Lebrette H, Hogbom M FEBS Lett. 2022 Feb 17. doi: 10.1002/1873-3468.14319. PMID:35175627<ref>PMID:35175627</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7qbp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Diamanti R]] | [[Category: Diamanti, R]] | ||
[[Category: Hogbom M]] | [[Category: Hogbom, M]] | ||
[[Category: Lebrette H]] | [[Category: Lebrette, H]] | ||
[[Category: Srinivas V]] | [[Category: Srinivas, V]] | ||
[[Category: Ferritin-like superfamily]] | |||
[[Category: Metalloprotein]] | |||
[[Category: Mn/fe cofactor]] | |||
[[Category: Oxidoreductase]] | |||
[[Category: R2-like ligand-binding oxidase]] | |||
[[Category: R2lox]] | |||
[[Category: Ribonucleotide reductase r2 subunit fold]] | |||
