1guc: Difference between revisions

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[[Image:1guc.gif|left|200px]]
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[[Image:1guc.png|left|200px]]


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{{STRUCTURE_1guc|  PDB=1guc  |  SCENE=  }}  
{{STRUCTURE_1guc|  PDB=1guc  |  SCENE=  }}  


'''SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES'''
===SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES===




==Overview==
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The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is unusually stable compared with other symmetric tandem GU mismatches and occurs in the P5 helix of the group I intron of Tetrahymena thermophila. The three-dimensional solution structure of (rGAGGUCUC)2 was determined using two-dimensional NMR and a simulated annealing protocol. The structure is remarkably similar to the A-DNA crystal structure of (dGGGGTCCC)2 [Kneale, G., Brown, T., &amp; Kennard, O. (1985) J. Mol. Biol. 186, 805-814] which contains the analogous motif [sequence: see text]. Incorporation of the [sequence: see text] motif has little effect on backbone torsion angles and helical parameters compared with standard A-form. The only significant departure from A-form is a slight overtwisting 5' of the G in the GU mismatch and a displacement of the mismatches toward the minor groove. Inspection of stacking patterns of this structure and comparison with symmetric tandem GT mismatches in A-DNA oligonucleotides from crystal structure data suggest that electrostatics are important in determining motif stability.
The line below this paragraph, {{ABSTRACT_PUBMED_8916893}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_8916893}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUC OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUC OCA].  


==Reference==
==Reference==
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[[Category: G:u mismatch]]
[[Category: G:u mismatch]]
[[Category: Ribonucleic acid]]
[[Category: Ribonucleic acid]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:01:03 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:05:38 2008''

Revision as of 03:05, 1 July 2008

File:1guc.png

Template:STRUCTURE 1guc

SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES

Template:ABSTRACT PUBMED 8916893

About this Structure

Full experimental information is available from OCA.

Reference

Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing., McDowell JA, Turner DH, Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:8916893

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