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| {{STRUCTURE_1gve| PDB=1gve | SCENE= }} | | {{STRUCTURE_1gve| PDB=1gve | SCENE= }} |
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| '''AFLATOXIN ALDEHYDE REDUCTASE (AKR7A1) FROM RAT LIVER'''
| | ===AFLATOXIN ALDEHYDE REDUCTASE (AKR7A1) FROM RAT LIVER=== |
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| ==Overview==
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| The structure of the rat liver aflatoxin dialdehyde reductase (AKR7A1) has been solved to 1.38-A resolution. Although it shares a similar alpha/beta-barrel structure with other members of the aldo-keto reductase superfamily, AKR7A1 is the first dimeric member to be crystallized. The crystal structure also reveals details of the ternary complex as one subunit of the dimer contains NADP(+) and the inhibitor citrate. Although the underlying catalytic mechanism appears similar to other aldo-keto reductases, the substrate-binding pocket contains several charged amino acids (Arg-231 and Arg-327) that distinguish it from previously characterized aldo-keto reductases with respect to size and charge. These differences account for the substrate specificity for 4-carbon acid-aldehydes such as succinic semialdehyde and 2-carboxybenzaldehyde as well as for the idiosyncratic substrate aflatoxin B(1) dialdehyde of this subfamily of enzymes. Structural differences between the AKR7A1 ternary complex and apoenzyme reveal a significant hinged movement of the enzyme involving not only the loops of the structure but also parts of the alpha/beta-barrel most intimately involved in cofactor binding. | | The line below this paragraph, {{ABSTRACT_PUBMED_11839745}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11839745 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11839745}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Aldo-keto reductase]] | | [[Category: Aldo-keto reductase]] |
| [[Category: Succinic semialdehyde oxidoreductase]] | | [[Category: Succinic semialdehyde oxidoreductase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:03:25 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:08:50 2008'' |
