Sandbox Reserved 1722: Difference between revisions
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==== ''Sodium Binding'' ==== | ==== ''Sodium Binding'' ==== | ||
The sodium site motif facilitates the conformational change of GPCR upon activation.<ref name="Katritch">PMID: 24767681</ref> A sodium molecule sits in the middle of the TM7 helices where it is stabilized by conserved residues aspartate (TM2), serine (TM2), and three water molecules. The sodium is able to make a salt bridge with the aspartate at this position. The sodium acts similar to a ball joint in which it allows for the TM helices be spread apart and induce larger conformational change upon binding. In MRGPRX2 this motif is only partially conserved. The aspartate (TM2) is conserved while the serine is replaced by a glycine.<ref name="Yang"/> This creates a less favorable environment for the stabilization of sodium. Currently, in crystallization structures no sodium has been seen at this site. Thus making it inconclusive on whether it plays a role in the conformational change to activate G-proteins upon binding to the receptor.<ref name="Yang"/> | The sodium site motif facilitates the conformational change of GPCR upon activation.<ref name="Katritch">PMID: 24767681</ref> A sodium molecule sits in the middle of the TM7 helices where it is stabilized by conserved residues aspartate (TM2), serine (TM2), and three water molecules. The sodium is able to make a salt bridge with the aspartate at this position. The sodium acts similar to a ball joint in which it allows for the TM helices be spread apart and induce larger conformational change upon binding. In MRGPRX2, this motif is only partially conserved. The aspartate (TM2) is conserved while the serine is replaced by a glycine.<ref name="Yang"/> This creates a less favorable environment for the stabilization of sodium due to serine being polar while glycine is nonpolar. Currently, in crystallization structures no sodium has been seen at this site. Thus making it inconclusive on whether it plays a role in the conformational change to activate G-proteins upon binding to the receptor.<ref name="Yang"/> | ||
== MRGPRX2 Signaling Pathway == | == MRGPRX2 Signaling Pathway == | ||
