7m0g: Difference between revisions
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==Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on Actin Filaments== | ==Magic Angle Spinning NMR Structure of Human Cofilin-2 Assembled on Actin Filaments== | ||
<StructureSection load='7m0g' size='340' side='right'caption='[[7m0g]]' scene=''> | <StructureSection load='7m0g' size='340' side='right'caption='[[7m0g]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full | <table><tr><td colspan='2'>[[7m0g]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M0G FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m0g OCA], [https://pdbe.org/7m0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m0g RCSB], [https://www.ebi.ac.uk/pdbsum/7m0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m0g ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m0g OCA], [https://pdbe.org/7m0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m0g RCSB], [https://www.ebi.ac.uk/pdbsum/7m0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m0g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Disease == | |||
[[https://www.uniprot.org/uniprot/COF2_HUMAN COF2_HUMAN]] Typical nemaline myopathy. The disease is caused by variants affecting the gene represented in this entry. | |||
== Function == | |||
[[https://www.uniprot.org/uniprot/COF2_HUMAN COF2_HUMAN]] Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed:19752190). It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).[UniProtKB:P45591]<ref>PMID:19752190</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Actin polymerization dynamics regulated by actin-binding proteins are essential for various cellular functions. The cofilin family of proteins are potent regulators of actin severing and filament disassembly. The structural basis for cofilin-isoform-specific severing activity is poorly understood as their high-resolution structures in complex with filamentous actin (F-actin) are lacking. Here, we present the atomic-resolution structure of the muscle-tissue-specific isoform, cofilin-2 (CFL2), assembled on ADP-F-actin, determined by magic-angle-spinning (MAS) NMR spectroscopy and data-guided molecular dynamics (MD) simulations. We observe an isoform-specific conformation for CFL2. This conformation is the result of a unique network of hydrogen bonding interactions within the alpha2 helix containing the non-conserved residue, Q26. Our results indicate F-site interactions that are specific between CFL2 and ADP-F-actin, revealing mechanistic insights into isoform-dependent F-actin disassembly. | |||
Magic angle spinning NMR structure of human cofilin-2 assembled on actin filaments reveals isoform-specific conformation and binding mode.,Kraus J, Russell RW, Kudryashova E, Xu C, Katyal N, Perilla JR, Kudryashov DS, Polenova T Nat Commun. 2022 Apr 19;13(1):2114. doi: 10.1038/s41467-022-29595-9. PMID:35440100<ref>PMID:35440100</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7m0g" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Kraus J]] | [[Category: Kraus, J]] | ||
[[Category: Perilla | [[Category: Perilla, J P]] | ||
[[Category: Polenova T]] | [[Category: Polenova, T]] | ||
[[Category: Xu C]] | [[Category: Xu, C]] | ||
[[Category: Actin filament binding]] | |||
[[Category: Actin filament severing]] | |||
[[Category: Contractile protein]] | |||
[[Category: Cytoskeletal assembly]] | |||
