3is1: Difference between revisions

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<StructureSection load='3is1' size='340' side='right'caption='[[3is1]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
<StructureSection load='3is1' size='340' side='right'caption='[[3is1]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3is1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stas1 Stas1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IS1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3is1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_saprophyticus_subsp._saprophyticus_ATCC_15305_=_NCTC_7292 Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IS1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3irp|3irp]], [[3irz|3irz]], [[3is0|3is0]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSP0135, uafA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342451 STAS1])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3is1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3is1 OCA], [https://pdbe.org/3is1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3is1 RCSB], [https://www.ebi.ac.uk/pdbsum/3is1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3is1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3is1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3is1 OCA], [https://pdbe.org/3is1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3is1 RCSB], [https://www.ebi.ac.uk/pdbsum/3is1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3is1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/UAFA_STAS1 UAFA_STAS1]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3is1 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3is1 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Staphylococci use cell wall-anchored proteins as adhesins to attach to host tissues. Staphylococcus saprophyticus, a uropathogenic species, has a unique cell wall-anchored protein, uro-adherence factor A (UafA), which shows erythrocyte binding activity. To investigate the mechanism of adhesion by UafA, we determined the crystal structure of the functional region of UafA at 1.5 A resolution. The structure was composed of three domains, designated as the N2, N3, and B domains, arranged in a triangular relative configuration. Hemagglutination inhibition assay with domain-truncated mutants indicated that both N and B domains were necessary for erythrocyte binding. Based on these results, a novel manner of ligand binding in which the B domain acts as a functional domain was proposed as the adhesion mechanism of S. saprophyticus.
Crystal structure of the functional region of Uro-adherence factor A from Staphylococcus saprophyticus reveals participation of the B domain in ligand binding.,Matsuoka E, Tanaka Y, Kuroda M, Shouji Y, Ohta T, Tanaka I, Yao M Protein Sci. 2011 Feb;20(2):406-16. doi: 10.1002/pro.573. PMID:21280131<ref>PMID:21280131</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3is1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Stas1]]
[[Category: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292]]
[[Category: Kuroda, M]]
[[Category: Kuroda M]]
[[Category: Matsuoka, E]]
[[Category: Matsuoka E]]
[[Category: Shouji, Y]]
[[Category: Shouji Y]]
[[Category: Tanaka, I]]
[[Category: Tanaka I]]
[[Category: Tanaka, Y]]
[[Category: Tanaka Y]]
[[Category: Yao, M]]
[[Category: Yao M]]
[[Category: Cell adhesion]]
[[Category: Cell wall]]
[[Category: Dev-igg fold]]
[[Category: Hemagglutinin]]
[[Category: Peptidoglycan-anchor]]
[[Category: Secreted]]
[[Category: Virulence]]
Retrieved from "https://proteopedia.org/3is1"