Sandbox Reserved 1722: Difference between revisions

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==== ''Toggle Switch'' ====

In β2AR, and other Class A GPCRs, a “toggle switch” of <scene name='90/904327/B2artoggleswitchyes/7'>Trp-286</scene> limits the proximity of the TM helices as tryptophan sterically occludes tight interaction. This results in a deep binding pocket for ligand binding. In contrast, in MRGPRX2 Trp-286 is replaced by <scene name='90/904327/Toggle_switch_gly_pt_2/3'>Gly-236</scene> <ref name="Cao"/> <ref name="Yang"/>. Glycine is a much smaller amino acid and thus allows the helices to close the base of the binding pocket. This causes MRGPRX2 to have a much shallower binding site and allows more promiscuous ligand binding. This can be seen in a shorter distance from the toggle switch to the ligand in β2AR (0.573 nm) compared to MRGPRX2 (1.389 nm).

In β2AR, and other Class A GPCRs, a “toggle switch” of <scene name='90/904327/B2artoggleswitchyes/8'>Trp-286</scene> limits the proximity of the TM helices as tryptophan sterically occludes tight interaction. This results in a deep binding pocket for ligand binding. In contrast, in MRGPRX2 Trp-286 is replaced by <scene name='90/904327/Toggle_switch_gly_pt_2/3'>Gly-236</scene> <ref name="Cao"/> <ref name="Yang"/>. Glycine is a much smaller amino acid and thus allows the helices to close the base of the binding pocket. This causes MRGPRX2 to have a much shallower binding site and allows more promiscuous ligand binding. This can be seen in a shorter distance from the toggle switch to the ligand in β2AR (0.573 nm) compared to MRGPRX2 (1.389 nm).

==== ''Disulfide bonds'' ====

Sandbox Reserved 1722: Difference between revisions

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@@ Line 24: / Line 24: @@
 ==== ''Toggle Switch'' ====
-In β2AR, and other Class A GPCRs, a “toggle switch” of <scene name='90/904327/B2artoggleswitchyes/7'>Trp-286</scene> limits the proximity of the TM helices as tryptophan sterically occludes tight interaction. This results in a deep binding pocket for ligand binding. In contrast, in MRGPRX2 Trp-286 is replaced by <scene name='90/904327/Toggle_switch_gly_pt_2/3'>Gly-236</scene> <ref name="Cao"/> <ref name="Yang"/>. Glycine is a much smaller amino acid and thus allows the helices to close the base of the binding pocket. This causes MRGPRX2 to have a much shallower binding site and allows more promiscuous ligand binding. This can be seen in a shorter distance from the toggle switch to the ligand in β2AR (0.573 nm) compared to MRGPRX2 (1.389 nm).
+In β2AR, and other Class A GPCRs, a “toggle switch” of <scene name='90/904327/B2artoggleswitchyes/8'>Trp-286</scene> limits the proximity of the TM helices as tryptophan sterically occludes tight interaction. This results in a deep binding pocket for ligand binding. In contrast, in MRGPRX2 Trp-286 is replaced by <scene name='90/904327/Toggle_switch_gly_pt_2/3'>Gly-236</scene> <ref name="Cao"/> <ref name="Yang"/>. Glycine is a much smaller amino acid and thus allows the helices to close the base of the binding pocket. This causes MRGPRX2 to have a much shallower binding site and allows more promiscuous ligand binding. This can be seen in a shorter distance from the toggle switch to the ligand in β2AR (0.573 nm) compared to MRGPRX2 (1.389 nm).
 ==== ''Disulfide bonds'' ====