BASIL2022GV3HDT: Difference between revisions

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===='''BLASTp'''====
===='''BLASTp'''====
Beginning with BLASTp<ref>2</ref>, we queried the FASTA sequence for our protein, PDB ID 3hdt. The alignment of our query sequence with the NK superfamily shows a high degree of overlap indicating this protein is likely a member. The importance of this is that the cytidylate kinase family is a member of the NK superfamily, supporting our claim that 3hdt is a cytidylate kinase. Also, the query hits show a couple cytidylate kinase-like family proteins almost completely allining with our protein 3HDT.
Beginning with BLASTp<ref>1</ref>, we queried the FASTA sequence for our protein, PDB ID 3hdt. The alignment of our query sequence with the NK superfamily shows a high degree of overlap indicating this protein is likely a member. The importance of this is that the cytidylate kinase family is a member of the NK superfamily, supporting our claim that 3hdt is a cytidylate kinase. Also, the query hits show a couple cytidylate kinase-like family proteins almost completely allining with our protein 3HDT.


[[Image:BLASTp image 1.png |600px| left | thumb | BLASTp Alignment showing a hit with cytidylate kinase-like family, part of the NK superfamily.]]
[[Image:BLASTp image 1.png |600px| left | thumb | BLASTp Alignment showing a hit with cytidylate kinase-like family, part of the NK superfamily.]]
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== References ==
== References ==
<references/>
<references/>
1. Holm L (2020) Using Dali for protein structure comparison. Methods Mol. Biol. 2112, 29-42.
1. National Center for Biotechnology Information (NCBI)[Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; [1988] – [cited 2022 April 23].


2. National Center for Biotechnology Information (NCBI)[Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; [1988] – [cited 2022 April 23].
2. Holm L (2020) Using Dali for protein structure comparison. Methods Mol. Biol. 2112, 29-42.


3. Pfam: The protein families database in 2021: J. Mistry, S. Chuguransky, L. Williams, M. Qureshi, G.A. Salazar, E.L.L. Sonnhammer, S.C.E. Tosatto, L. Paladin, S. Raj, L.J. Richardson, R.D. Finn, A. Bateman
3. Pfam: The protein families database in 2021: J. Mistry, S. Chuguransky, L. Williams, M. Qureshi, G.A. Salazar, E.L.L. Sonnhammer, S.C.E. Tosatto, L. Paladin, S. Raj, L.J. Richardson, R.D. Finn, A. Bateman