Ceramidase: Difference between revisions

Revision as of 16:24, 30 April 2022

Function

CerN is an enzyme that catalyzes the cleavage of the Sphingolipid Ceramide at the N-acyl linkage, producing sphingosine and a fatty acid.^[1] ^[2]

Refs ^[1], ^[3] , ^[4]

Disease

p^[1]

Relevance

p

Structural highlights

This is a sample scene created with SAT to color by Group, and another to make a transparent representation of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.active site

2zxc is a 2 chain structure with sequence from "bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	2ED, DMS, FMT, MG, ZN
Activity:	Ceramidase, with EC number 3.5.1.23
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

↑ ^1.0 ^1.1 ^1.2 Okino N, Tani M, Imayama S, Ito M. Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa. J Biol Chem. 1998 Jun 5;273(23):14368-73. PMID:9603946
↑ Inoue T, Okino N, Kakuta Y, Hijikata A, Okano H, Goda HM, Tani M, Sueyoshi N, Kambayashi K, Matsumura H, Kai Y, Ito M. Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase. J Biol Chem. 2009 Apr 3;284(14):9566-77. Epub 2008 Dec 16. PMID:19088069 doi:10.1074/jbc.M808232200
↑ Inoue T, Okino N, Kakuta Y, Hijikata A, Okano H, Goda HM, Tani M, Sueyoshi N, Kambayashi K, Matsumura H, Kai Y, Ito M. Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase. J Biol Chem. 2009 Apr 3;284(14):9566-77. Epub 2008 Dec 16. PMID:19088069 doi:10.1074/jbc.M808232200
↑ Kita K, Okino N, Ito M. Reverse hydrolysis reaction of a recombinant alkaline ceramidase of Pseudomonas aeruginosa. Biochim Biophys Acta. 2000 May 31;1485(2-3):111-20. doi:, 10.1016/s1388-1981(00)00029-9. PMID:10832092 doi:https://dx.doi.org/10.1016/s1388-1981(00)00029-9

References

Proteopedia Page Contributors and Editors (what is this?)

Jacob R. Mackinder, Michal Harel, Jaime Prilusky

[Okino-1] 1.0 ^1.1 ^1.2 Okino N, Tani M, Imayama S, Ito M. Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa. J Biol Chem. 1998 Jun 5;273(23):14368-73. PMID:9603946

[2] Inoue T, Okino N, Kakuta Y, Hijikata A, Okano H, Goda HM, Tani M, Sueyoshi N, Kambayashi K, Matsumura H, Kai Y, Ito M. Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase. J Biol Chem. 2009 Apr 3;284(14):9566-77. Epub 2008 Dec 16. PMID:19088069 doi:10.1074/jbc.M808232200

[Inoue-3] Inoue T, Okino N, Kakuta Y, Hijikata A, Okano H, Goda HM, Tani M, Sueyoshi N, Kambayashi K, Matsumura H, Kai Y, Ito M. Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase. J Biol Chem. 2009 Apr 3;284(14):9566-77. Epub 2008 Dec 16. PMID:19088069 doi:10.1074/jbc.M808232200

[Reverse-4] Kita K, Okino N, Ito M. Reverse hydrolysis reaction of a recombinant alkaline ceramidase of Pseudomonas aeruginosa. Biochim Biophys Acta. 2000 May 31;1485(2-3):111-20. doi:, 10.1016/s1388-1981(00)00029-9. PMID:10832092 doi:https://dx.doi.org/10.1016/s1388-1981(00)00029-9

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@@ Line 3: / Line 3: @@
 '''CerN''' is an enzyme that catalyzes the cleavage of the [https://en.wikipedia.org/wiki/Sphingolipid Sphingolipid] <scene name='91/910024/Ceramide/3'>Ceramide</scene> at the <scene name='91/910024/Ceramide18/1'>N-acyl linkage</scene>, producing <scene name='91/910024/Ceramide/4'>sphingosine and a fatty acid</scene>.<ref name="Okino">PMID:9603946</ref> <ref>PMID:19088069</ref>
-siteZn2+
+Refs <ref name="Okino">PMID:9603946</ref>, <ref name="Inoue">PMID:19088069</ref> , <ref name="Reverse">PMID:10832092</ref>
 == Disease ==

Ceramidase: Difference between revisions

Revision as of 16:24, 30 April 2022

Contents

Function

Disease

Relevance

Structural highlights

References

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