Ceramidase: Difference between revisions

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CerN consists of two domains: a <scene name='91/910024/Domains/1'>catalytic domain near the N-terminal and an immunoglobulin-fold domain near the C-terminal</scene>.<ref name="Inoue">PMID:19088069</ref> Three β-sheets, each formed from four β-strands, compose a β-prism fold at the center of the N-terminal domain.<ref name="Inoue">PMID:19088069</ref> Surrounding the β-prism fold are 11 α-helices, forming an <scene name='91/910024/Bprism/3'>α+ β 2-layer sandwich fold</scene>.<ref name="Inoue">PMID:19088069</ref> The immunoglobulin-like C-terminal domain is composed of two β-sheets, containing four β-strands each, forming a <scene name='91/910024/Igfold/1'>β-sandwich fold</scene>.<ref name="Inoue">PMID:19088069</ref> Between the N- and C-terminal domains is a magnesium/calcium ion binding site that links together the two domains.<ref name="Inoue">PMID:19088069</ref> His37, Asp579, Asp581, and Thr854 interact with divalent cations within the <scene name='91/910024/Mg_bs/1'>magnesium/calcium ion binding site</scene>.<ref name="Inoue">PMID:19088069</ref> A <scene name='91/910024/Zinc_bs/3'>second metal-binding site containing a zinc ion</scene> is located within the N-terminal domain active site, where it is coordinated by His97, His204, Glu411, and a water molecule.<ref name="Inoue">PMID:19088069</ref>  
CerN consists of two domains: a <scene name='91/910024/Domains/1'>catalytic domain near the N-terminal and an immunoglobulin-fold domain near the C-terminal</scene>.<ref name="Inoue">PMID:19088069</ref> Three β-sheets, each formed from four β-strands, compose a β-prism fold at the center of the N-terminal domain.<ref name="Inoue">PMID:19088069</ref> Surrounding the β-prism fold are 11 α-helices, forming an <scene name='91/910024/Bprism/3'>α+ β 2-layer sandwich fold</scene>.<ref name="Inoue">PMID:19088069</ref> The immunoglobulin-like C-terminal domain is composed of two β-sheets, containing four β-strands each, forming a <scene name='91/910024/Igfold/1'>β-sandwich fold</scene>.<ref name="Inoue">PMID:19088069</ref> Between the N- and C-terminal domains is a magnesium/calcium ion binding site that links together the two domains.<ref name="Inoue">PMID:19088069</ref> His37, Asp579, Asp581, and Thr854 interact with divalent cations within the <scene name='91/910024/Mg_bs/1'>magnesium/calcium ion binding site</scene>.<ref name="Inoue">PMID:19088069</ref> A <scene name='91/910024/Zinc_bs/3'>second metal-binding site containing a zinc ion</scene> is located within the N-terminal domain active site, where it is coordinated by His97, His204, Glu411, and a water molecule.<ref name="Inoue">PMID:19088069</ref>  


 
Full crystallographic information for 2zxc (Closed) is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZXC OCA].Full crystallographic information for 2zws (Open) is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZWS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2ZXC FirstGlance]. <br>
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
 
<table><tr><td colspan='2'>[[2zxc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZXC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2ZXC FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2ED:N-[(1R,2R,3E)-2-HYDROXY-1-(HYDROXYMETHYL)HEPTADEC-3-EN-1-YL]ACETAMIDE'>2ED</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2ED:N-[(1R,2R,3E)-2-HYDROXY-1-(HYDROXYMETHYL)HEPTADEC-3-EN-1-YL]ACETAMIDE'>2ED</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ceramidase Ceramidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.23 3.5.1.23] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ceramidase Ceramidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.23 3.5.1.23] </span></td></tr>
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