7zc0: Difference between revisions

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'''Unreleased structure'''


The entry 7zc0 is ON HOLD  until Paper Publication
==4,6-alpha-glucanotransferase GtfC from Geobacillus 12AMOR1==
<StructureSection load='7zc0' size='340' side='right'caption='[[7zc0]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7zc0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus Geobacillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZC0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zc0 OCA], [https://pdbe.org/7zc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7zc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zc0 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
GtfC-type 4,6-alpha-glucanotransferase (alpha-GT) enzymes from Glycoside Hydrolase Family 70 (GH70) are of interest for the modification of starch into low-glycemic index food ingredients. Compared to the related GH70 GtfB-type alpha-GTs, found exclusively in lactic acid bacteria (LAB), GtfCs occur in non-LAB, share low sequence identity, lack circular permutation of the catalytic domain, and feature a single-segment auxiliary domain IV and auxiliary C-terminal domains. Despite these differences, the first crystal structure of a GtfC, GbGtfC-DeltaC from Geobacillus 12AMOR1, and the first one representing a non-permuted GH70 enzyme, reveals high structural similarity in the core domains with most GtfBs, featuring a similar tunneled active site. We propose that GtfC (and related GtfD) enzymes evolved from starch-degrading alpha-amylases from GH13 by acquiring alpha-1,6 transglycosylation capabilities, before the events that resulted in circular permutation of the catalytic domain observed in other GH70 enzymes (glucansucrases, GtfB-type alpha-GTs). AlphaFold modeling and sequence alignments suggest that the GbGtfC structure represents the GtfC subfamily, although it has a so far unique alternating alpha-1,4/alpha-1,6 product specificity, likely determined by residues near acceptor binding subsites +1/+2.


Authors:  
Crystal Structure of 4,6-alpha-Glucanotransferase GtfC-DeltaC from Thermophilic Geobacillus 12AMOR1: Starch Transglycosylation in Non-Permuted GH70 Enzymes.,Pijning T, Te Poele EM, de Leeuw TC, Guskov A, Dijkhuizen L J Agric Food Chem. 2022 Nov 28. doi: 10.1021/acs.jafc.2c06394. PMID:36442227<ref>PMID:36442227</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 7zc0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus]]
[[Category: Large Structures]]
[[Category: Dijkhuizen L]]
[[Category: Guskov A]]
[[Category: Pijning T]]
[[Category: Te Poele EM]]
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