BASIL2022GV3HDT: Difference between revisions

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[[Image:Coupled assay.png |400px|left| thumb | Coupled kinase assay diagram with enzymes shown in color and phosphates in yellow. Phosphorylation of dCMP is measured indirectly through the conversion of NADH to NAD+. Background hydrolysis of NADH is measured and subtracted from the conversion rate in the presence of dCMP before calculating specific activity values.]]
[[Image:Coupled assay.png |400px|left| thumb | Coupled kinase assay diagram with enzymes shown in color and phosphates in yellow. Phosphorylation of dCMP is measured indirectly through the conversion of NADH to NAD+.]]


[[Image:Updated Kinetics Table 5.4.22 3.PNG‎ |500px|center| thumb | Data table with kinetics information on all eight coupled kinase assays with 3HDT and dCMP along with their specific activity values.]]
[[Image:Updated Kinetics Table 5.4.22 3.PNG‎ |500px|center| thumb | Specific activity values and other data from all coupled kinase assays with 3HDT and dCMP.]]
 


Background hydrolysis of NADH was measured and subtracted from the conversion rate in the presence of dCMP before calculating specific activity values.


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