BASIL2022GV3HDT: Difference between revisions
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== Conclusion | == Conclusion & Future Experiments == | ||
Our protein, 3HDT, was confirmed to be present using SDS-PAGE and showed activity during coupled kinase assays. While this confirms that 3HDT is a kinase, the true substrate was likely not dCMP due to the low specific activity values. Further research should be done with molecules such as TMP and GMP in the future to narrow down potential nucleotide substrates or elucidate other types of compounds to be considered as ligands for 3HDT. Additionally, in the future we would like to repeat the protein purification process to try and get a higher protein concentration than what we achieved as the low concentration may have affected our coupled kinase assay results. | Our protein, 3HDT, was confirmed to be present using SDS-PAGE and showed activity during coupled kinase assays. While this confirms that 3HDT is a kinase, the true substrate was likely not dCMP due to the low specific activity values. Further research should be done with molecules such as TMP and GMP in the future to narrow down potential nucleotide substrates or elucidate other types of compounds to be considered as ligands for 3HDT. Additionally, in the future we would like to repeat the protein purification process to try and get a higher protein concentration than what we achieved as the low concentration may have affected our coupled kinase assay results. | ||
