1h56: Difference between revisions
New page: left|200px<br /> <applet load="1h56" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h56, resolution 3.0Å" /> '''STRUCTURAL AND BIOCH... |
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==About this Structure== | ==About this Structure== | ||
1H56 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H56 OCA]]. | 1H56 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4]]. Structure known Active Site: MGA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H56 OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Proteus vulgaris]] | [[Category: Proteus vulgaris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Type II site-specific deoxyribonuclease]] | |||
[[Category: Athanasiadis, A.]] | [[Category: Athanasiadis, A.]] | ||
[[Category: Jeltsch, A.]] | [[Category: Jeltsch, A.]] | ||
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[[Category: type ii restriction endonuclease]] | [[Category: type ii restriction endonuclease]] | ||
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Revision as of 09:30, 30 October 2007
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STRUCTURAL AND BIOCHEMICAL CHARACTERIZATION OF A NEW MAGNESIUM ION BINDING SITE NEAR TYR94 IN THE RESTRICTION ENDONUCLEASE PVUII
Overview
We have determined the crystal structure of the PvuII endonuclease in the, presence of Mg(2+). According to the structural data, divalent metal ion, binding in the PvuII subunits is highly asymmetric. The PvuII-Mg(2+), complex has two distinct metal ion binding sites, one in each monomer. One, site is formed by the catalytic residues Asp58 and Glu68, and has, extensive similarities to a catalytically important site found in all, structurally examined restriction endonucleases. The other binding site is, located in the other monomer, in the immediate vicinity of the hydroxyl, group of Tyr94; it has no analogy to metal ion binding sites found so far, in restriction endonucleases. To assign the number of metal ions involved, and to better understand the role of Mg(2+) binding to Tyr94 for ... [(full description)]
About this Structure
1H56 is a [Single protein] structure of sequence from [Proteus vulgaris] with MG as [ligand]. Active as [Type II site-specific deoxyribonuclease], with EC number [3.1.21.4]. Structure known Active Site: MGA. Full crystallographic information is available from [OCA].
Reference
Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII., Spyridaki A, Matzen C, Lanio T, Jeltsch A, Simoncsits A, Athanasiadis A, Scheuring-Vanamee E, Kokkinidis M, Pingoud A, J Mol Biol. 2003 Aug 8;331(2):395-406. PMID:12888347
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