3p26: Difference between revisions

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<StructureSection load='3p26' size='340' side='right'caption='[[3p26]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='3p26' size='340' side='right'caption='[[3p26]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3p26]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P26 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3p26]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P26 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3p27|3p27]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HBS1, YKR084C, YKR404 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p26 OCA], [https://pdbe.org/3p26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p26 RCSB], [https://www.ebi.ac.uk/pdbsum/3p26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p26 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p26 OCA], [https://pdbe.org/3p26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p26 RCSB], [https://www.ebi.ac.uk/pdbsum/3p26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p26 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/HBS1_YEAST HBS1_YEAST]] Involved in protein translation. Together with DOM34, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs.<ref>PMID:16554824</ref> 
[https://www.uniprot.org/uniprot/HBS1_YEAST HBS1_YEAST] Involved in protein translation. Together with DOM34, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs.<ref>PMID:16554824</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Eukaryotic cells have several quality control pathways that rely on translation to detect and degrade defective RNAs. Dom34 and Hbs1 are two proteins that are related to translation termination factors and are involved in no-go decay (NGD) and nonfunctional 18S ribosomal RNA (rRNA) decay (18S NRD) pathways that eliminate RNAs that cause strong ribosomal stalls. Here we present the structure of Hbs1 with and without GDP and a low-resolution model of the Dom34-Hbs1 complex. This complex mimics complexes of the elongation factor and transfer RNA or of the translation termination factors eRF1 and eRF3, supporting the idea that it binds to the ribosomal A-site. We show that nucleotide binding by Hbs1 is essential for NGD and 18S NRD. Mutations in Hbs1 that disrupted the interaction between Dom34 and Hbs1 strongly impaired NGD but had almost no effect on 18S NRD. Hence, NGD and 18S NRD could be genetically uncoupled, suggesting that mRNA and rRNA in a stalled translation complex may not always be degraded simultaneously.
 
Dissection of Dom34-Hbs1 reveals independent functions in two RNA quality control pathways.,van den Elzen AM, Henri J, Lazar N, Gas ME, Durand D, Lacroute F, Nicaise M, van Tilbeurgh H, Seraphin B, Graille M Nat Struct Mol Biol. 2010 Dec;17(12):1446-52. Epub 2010 Nov 21. PMID:21102444<ref>PMID:21102444</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3p26" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 18824]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Durand, D]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Elzen, A van den]]
[[Category: Durand D]]
[[Category: Gas, M E]]
[[Category: Gas ME]]
[[Category: Graille, M]]
[[Category: Graille M]]
[[Category: Henri, J]]
[[Category: Henri J]]
[[Category: Lacroute, F]]
[[Category: Lacroute F]]
[[Category: Lazar, N]]
[[Category: Lazar N]]
[[Category: Nicaise, M]]
[[Category: Nicaise M]]
[[Category: Sraphin, B]]
[[Category: Sraphin B]]
[[Category: Tilbeurgh, H van]]
[[Category: Van Tilbeurgh H]]
[[Category: Structural genomic]]
[[Category: Van den Elzen A]]
[[Category: Beta-barrel]]
[[Category: Dom34]]
[[Category: Gtp/gdp binding domain]]
[[Category: Signaling protein]]
[[Category: Translational gtpase]]
[[Category: Ysg]]

Latest revision as of 10:38, 21 February 2024

Crystal structure of S. cerevisiae Hbs1 protein (apo-form), a translational GTPase involved in RNA quality control pathways and interacting with Dom34/Pelota

3p26, resolution 2.50Å

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