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| ==Structure of light-adapted AsLOV2 Q513L== | | ==Structure of light-adapted AsLOV2 Q513L== |
| <StructureSection load='7ph0' size='340' side='right'caption='[[7ph0]], [[Resolution|resolution]] 0.98Å' scene=''> | | <StructureSection load='7ph0' size='340' side='right'caption='[[7ph0]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[7ph0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PH0 FirstGlance]. <br> | | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PH0 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph0 OCA], [https://pdbe.org/7ph0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph0 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph0 OCA], [https://pdbe.org/7ph0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph0 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| In nature as in biotechnology, light-oxygen-voltage photoreceptors perceive blue light to elicit spatiotemporally defined cellular responses. Photon absorption drives thioadduct formation between a conserved cysteine and the flavin chromophore. An equally conserved, proximal glutamine processes the resultant flavin protonation into downstream hydrogen-bond rearrangements. Here, we report that this glutamine, long deemed essential, is generally dispensable. In its absence, several light-oxygen-voltage receptors invariably retained productive, if often attenuated, signaling responses. Structures of a light-oxygen-voltage paradigm at around 1 A resolution revealed highly similar light-induced conformational changes, irrespective of whether the glutamine is present. Naturally occurring, glutamine-deficient light-oxygen-voltage receptors likely serve as bona fide photoreceptors, as we showcase for a diguanylate cyclase. We propose that without the glutamine, water molecules transiently approach the chromophore and thus propagate flavin protonation downstream. Signaling without glutamine appears intrinsic to light-oxygen-voltage receptors, which pertains to biotechnological applications and suggests evolutionary descendance from redox-active flavoproteins.
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| Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine.,Dietler J, Gelfert R, Kaiser J, Borin V, Renzl C, Pilsl S, Ranzani AT, Garcia de Fuentes A, Gleichmann T, Diensthuber RP, Weyand M, Mayer G, Schapiro I, Moglich A Nat Commun. 2022 May 12;13(1):2618. doi: 10.1038/s41467-022-30252-4. PMID:35552382<ref>PMID:35552382</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 7ph0" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Gelfert, R]] | | [[Category: Gelfert R]] |
| [[Category: Moeglich, A]] | | [[Category: Moeglich A]] |
| [[Category: Weyand, M]] | | [[Category: Weyand M]] |
| [[Category: Flavin]]
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| [[Category: Light-oxygen-voltage]]
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| [[Category: Optogenetic]]
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| [[Category: Photoreceptor]]
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| [[Category: Phototropin]]
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| [[Category: Signaling protein]]
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