7xsh: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of ClAgl29B bound with L-glucose== | |||
<StructureSection load='7xsh' size='340' side='right'caption='[[7xsh]], [[Resolution|resolution]] 1.71Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7xsh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cecembia_lonarensis_LW9 Cecembia lonarensis LW9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSH FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=Z8T:beta-L-glucopyranose'>Z8T</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xsh OCA], [https://pdbe.org/7xsh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xsh RCSB], [https://www.ebi.ac.uk/pdbsum/7xsh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xsh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/K1KV82_9BACT K1KV82_9BACT] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Glucose, a common monosaccharide in nature, is dominated by the d-enantiomer. Meanwhile, the discovery of l-glucose-utilizing bacteria and the elucidation of their metabolic pathways 10 years ago suggests that l-glucose exists naturally. Most carbohydrates exist as glycosides rather than monosaccharides; therefore, we expected that nature also contains l-glucosides. Sequence analysis within glycoside hydrolase family 29 led us to identify two alpha-l-glucosidases, ClAgl29A and ClAgl29B, derived from Cecembia lonarensis LW9. ClAgl29A and ClAgl29B exhibited higher K (m), k (cat), and k (cat)/K (m) values for p-nitrophenyl alpha-l-glucoside than that for p-nitrophenyl alpha-l-fucoside. Structural analysis of ClAgl29B in complex with l-glucose showed that these enzymes have an active-site pocket that preferentially binds alpha-l-glucoside, but excludes alpha-l-fucoside. These results suggest that ClAgl29A and ClAgl29B evolved to hydrolyze alpha-l-glucoside, implying the existence of alpha-l-glucoside in nature. Furthermore, alpha-l-glucosidic linkages (alpha-l-Glc-(1 --> 3)-l-Glc, alpha-l-Glc-(1 --> 2)-l-Glc, and alpha-l-Glc-(1 --> 6)-l-Glc) were synthesized by the transglucosylation activity of ClAgl29A and ClAgl29B. We believe that this study will lead to new research on alpha-l-glucosides, including determining the physiological effects on humans, and the discovery of novel alpha-l-glucoside-related enzymes. | |||
Discovery of alpha-l-Glucosidase Raises the Possibility of alpha-l-Glucosides in Nature.,Shishiuchi R, Kang H, Tagami T, Ueda Y, Lang W, Kimura A, Okuyama M ACS Omega. 2022 Dec 9;7(50):47411-47423. doi: 10.1021/acsomega.2c06991. , eCollection 2022 Dec 20. PMID:36570207<ref>PMID:36570207</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7xsh" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cecembia lonarensis LW9]] | |||
[[Category: Large Structures]] | |||
[[Category: Kang H]] | |||
[[Category: Okuyama M]] | |||
[[Category: Shishiuchi R]] | |||
[[Category: Tagami T]] | |||
