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| [[Image:1h99.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1h99| PDB=1h99 | SCENE= }} | | {{STRUCTURE_1h99| PDB=1h99 | SCENE= }} |
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| '''PRD OF LICT ANTITERMINATOR FROM BACILLUS SUBTILIS'''
| | ===PRD OF LICT ANTITERMINATOR FROM BACILLUS SUBTILIS=== |
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| ==Overview==
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| The transcriptional antiterminator protein LicT regulates the expression of Bacillus subtilis operons involved in beta-glucoside metabolism. It belongs to a newly characterized family of bacterial regulators whose activity is controlled by the phosphoenolpyruvate:sugar phosphotransferase system (PTS). LicT contains an N-terminal RNA-binding domain (56 residues), and a PTS regulation domain (PRD, 221 residues) that is phosphorylated on conserved histidines in response to substrate availability. Replacement of both His207 and His269 with a negatively charged residue (aspartic acid) led to a highly active LicT variant that no longer responds to either induction or catabolite repression signals from the PTS. In contrast to wild type, the activated mutant form of the LicT regulatory domain crystallized easily and provided the first structure of a PRD, determined at 1.55 A resolution. The structure is a homodimer, each monomer containing two analogous alpha-helical domains. The phosphorylation sites are totally buried at the dimer interface and hence inaccessible to phosphorylating partners. The structure suggests important tertiary and quaternary rearrangements upon LicT activation, which could be communicated from the protein C-terminal end up to the RNA-binding domain. | | The line below this paragraph, {{ABSTRACT_PUBMED_11447120}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11447120 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11447120}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Pts regulatory domain]] | | [[Category: Pts regulatory domain]] |
| [[Category: Transcriptional antiterminator]] | | [[Category: Transcriptional antiterminator]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:35:40 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:56:31 2008'' |
