8d9e: Difference between revisions

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~~'''Unreleased structure'''~~

The ~~entry 8d9e~~ is ~~ON HOLD~~

==gRAMP-match PFS target==

== Structural highlights ==

<table><tr><td colspan='2'>[[8d9e]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D9E FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d9e OCA], [https://pdbe.org/8d9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d9e RCSB], [https://www.ebi.ac.uk/pdbsum/8d9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d9e ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]

== Publication Abstract from PubMed ==

The Type III-E RNA-targeting effector complex (gRAMP/Cas7-11) is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here we use cryo-electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms. Target-guide pairing extending into the 5' region of the guide RNA displaces a gating loop in gRAMP, which triggers an extensive conformational relay that allosterically aligns the protease catalytic dyad and opens an amino acid sidechain-binding pocket. We further define Csx30 as the endogenous protein substrate that is site-specifically proteolyzed by RNA-activated Craspase. This protease activity is switched off by target RNA cleavage by gRAMP, and is not activated by RNA targets containing a matching protospacer flanking sequence. We thus conclude that Craspase is a target RNA-activated protease with self-regulatory capacity.

~~Authors~~:

Craspase is a CRISPR RNA-guided, RNA-activated protease.,Hu C, van Beljouw SPB, Nam KH, Schuler G, Ding F, Cui Y, Rodriguez-Molina A, Haagsma AC, Valk M, Pabst M, Brouns SJJ, Ke A Science. 2022 Aug 25:eadd5064. doi: 10.1126/science.add5064. PMID:36007061<ref>PMID:36007061</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Candidatus Scalindua brodae]]

[[Category: Large Structures]]

[[Category: Hu C]]

[[Category: Ke A]]

[[Category: Nam KH]]

[[Category: Schuler G]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8d9e is ON HOLD
+==gRAMP-match PFS target==
+<StructureSection load='8d9e' size='340' side='right'caption='[[8d9e]], [[Resolution|resolution]] 3.76&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8d9e]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Scalindua_brodae Candidatus Scalindua brodae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D9E FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d9e OCA], [https://pdbe.org/8d9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d9e RCSB], [https://www.ebi.ac.uk/pdbsum/8d9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d9e ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0B0EGF3_9BACT A0A0B0EGF3_9BACT]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Type III-E RNA-targeting effector complex (gRAMP/Cas7-11) is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here we use cryo-electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms. Target-guide pairing extending into the 5' region of the guide RNA displaces a gating loop in gRAMP, which triggers an extensive conformational relay that allosterically aligns the protease catalytic dyad and opens an amino acid sidechain-binding pocket. We further define Csx30 as the endogenous protein substrate that is site-specifically proteolyzed by RNA-activated Craspase. This protease activity is switched off by target RNA cleavage by gRAMP, and is not activated by RNA targets containing a matching protospacer flanking sequence. We thus conclude that Craspase is a target RNA-activated protease with self-regulatory capacity.
-Authors:
+Craspase is a CRISPR RNA-guided, RNA-activated protease.,Hu C, van Beljouw SPB, Nam KH, Schuler G, Ding F, Cui Y, Rodriguez-Molina A, Haagsma AC, Valk M, Pabst M, Brouns SJJ, Ke A Science. 2022 Aug 25:eadd5064. doi: 10.1126/science.add5064. PMID:36007061<ref>PMID:36007061</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8d9e" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Candidatus Scalindua brodae]]
+[[Category: Large Structures]]
+[[Category: Hu C]]
+[[Category: Ke A]]
+[[Category: Nam KH]]
+[[Category: Schuler G]]