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| {{STRUCTURE_1hm6| PDB=1hm6 | SCENE= }} | | {{STRUCTURE_1hm6| PDB=1hm6 | SCENE= }} |
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| '''X-RAY STRUCTURE OF FULL-LENGTH ANNEXIN 1'''
| | ===X-RAY STRUCTURE OF FULL-LENGTH ANNEXIN 1=== |
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| ==Overview==
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| Annexins comprise a multigene family of Ca2+ and phospholipid- binding proteins. They consist of a conserved C-terminal or core domain that confers Ca2+-dependent phospholipid binding and an N-terminal domain that is variable in sequence and length and responsible for the specific properties of each annexin. Crystal structures of various annexin core domains have revealed a high degree of similarity. From these and other studies it is evident that the core domain harbors the calcium-binding sites that interact with the phospholipid headgroups. However, no structure has been reported of an annexin with a complete N-terminal domain. We have now solved the crystal structure of such a full-length annexin, annexin 1. Annexin 1 is active in membrane aggregation and its refined 1.8 A structure shows an alpha-helical N-terminal domain connected to the core domain by a flexible linker. It is surprising that the two alpha-helices present in the N-terminal domain of 41 residues interact intimately with the core domain, with the amphipathic helix 2-12 of the N-terminal domain replacing helix D of repeat III of the core. In turn, helix D is unwound into a flap now partially covering the N-terminal helix. Implications for membrane aggregation will be discussed and a model of aggregation based on the structure will be presented.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11178908}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11178908 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11178908}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Calcium-free form]] | | [[Category: Calcium-free form]] |
| [[Category: Full-length protein comprising protein core and n-terminal domain]] | | [[Category: Full-length protein comprising protein core and n-terminal domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:00:23 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:23:27 2008'' |
