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| [[Image:1hme.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1hme| PDB=1hme | SCENE= }} | | {{STRUCTURE_1hme| PDB=1hme | SCENE= }} |
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| '''STRUCTURE OF THE HMG BOX MOTIF IN THE B-DOMAIN OF HMG1'''
| | ===STRUCTURE OF THE HMG BOX MOTIF IN THE B-DOMAIN OF HMG1=== |
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| ==Overview==
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| The conserved, abundant chromosomal protein HMG1 consists of two highly homologous, folded, basic DNA-binding domains, each of approximately 80 amino acid residues, and an acidic C-terminal tail. Each folded domain represents an 'HMG box', a sequence motif recently recognized in certain sequence-specific DNA-binding proteins and which also occurs in abundant HMG1-like proteins that bind to DNA without sequence specificity. The HMG box is defined by a set of highly conserved residues (most distinctively aromatic and basic) and appears to define a novel DNA-binding structural motif. We have expressed the HMG box region of the B-domain of rat HMG1 (residues 88-164 of the intact protein) in Escherichia coli and we describe here the determination of its structure by 2D 1H-NMR spectroscopy. There are three alpha-helices (residues 13-29, 34-48 and 50-74), which together account for approximately 75% of the total residues and contain many of the conserved basic and aromatic residues. Strikingly, the molecule is L-shaped, the angle of approximately 80 degrees between the two arms being defined by a cluster of conserved, predominantly aromatic, residues. The distinctive shape of the HMG box motif, which is distinct from hitherto characterized DNA-binding motifs, may be significant in relation to its recognition of four-way DNA junctions. | | The line below this paragraph, {{ABSTRACT_PUBMED_8467791}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8467791 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_8467791}} |
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| ==About this Structure== | | ==About this Structure== |
| 1HME is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HME OCA]. | | 1HME is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HME OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Weir, H M.]] | | [[Category: Weir, H M.]] |
| [[Category: Dna-binding]] | | [[Category: Dna-binding]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:00:52 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:23:55 2008'' |
