3ufk: Difference between revisions

Latest revision as of 09:07, 15 November 2023

Crystal structure of UndA complexed with Iron Nitrilotriacetate

Structural highlights

3ufk is a 1 chain structure with sequence from Shewanella sp. HRCR_06. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Ligands:	CA, FE, GOL, HEC, MG, NTA, O
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

F8UWD6_9GAMM

Publication Abstract from PubMed

Members of the genus Shewanella translocate deca- or undeca-heme cytochromes to the external cell surface thus enabling respiration using extracellular minerals and polynuclear Fe(III) chelates. The high resolution structure of the first undeca-heme outer membrane cytochrome, UndA, reveals a crossed heme chain with four potential electron ingress/egress sites arranged within four domains. Sequence and structural alignment of UndA and the deca-heme MtrF reveals the extra heme of UndA is inserted between MtrF hemes 6 and 7. The remaining UndA hemes can be superposed over the heme chain of the decaheme MtrF, suggesting that a ten heme core is conserved between outer membrane cytochromes. The UndA structure has also been crystallographically resolved in complex with substrates, an Fe(III)-nitrilotriacetate dimer or an Fe(III)-citrate trimer. The structural resolution of these UndA-Fe(III)-chelate complexes provides a rationale for previous kinetic measurements on UndA and other outer membrane cytochromes.

The Crystal Structure of the Extracellular 11-heme Cytochrome UndA Reveals a Conserved 10-heme Motif and Defined Binding Site for Soluble Iron Chelates.,Edwards MJ, Hall A, Shi L, Fredrickson JK, Zachara JM, Butt JN, Richardson DJ, Clarke TA Structure. 2012 Jul 3;20(7):1275-84. Epub 2012 Jun 7. PMID:22682743^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Edwards MJ, Hall A, Shi L, Fredrickson JK, Zachara JM, Butt JN, Richardson DJ, Clarke TA. The Crystal Structure of the Extracellular 11-heme Cytochrome UndA Reveals a Conserved 10-heme Motif and Defined Binding Site for Soluble Iron Chelates. Structure. 2012 Jul 3;20(7):1275-84. Epub 2012 Jun 7. PMID:22682743 doi:10.1016/j.str.2012.04.016

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OCA

[1] Edwards MJ, Hall A, Shi L, Fredrickson JK, Zachara JM, Butt JN, Richardson DJ, Clarke TA. The Crystal Structure of the Extracellular 11-heme Cytochrome UndA Reveals a Conserved 10-heme Motif and Defined Binding Site for Soluble Iron Chelates. Structure. 2012 Jul 3;20(7):1275-84. Epub 2012 Jun 7. PMID:22682743 doi:10.1016/j.str.2012.04.016

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='3ufk' size='340' side='right'caption='[[3ufk]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ufk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_sp._hrcr-6 Shewanella sp. hrcr-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UFK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ufk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_sp._HRCR_06 Shewanella sp. HRCR_06]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UFK FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NTA:NITRILOTRIACETIC+ACID'>NTA</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ucp|3ucp]], [[3ufh|3ufh]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NTA:NITRILOTRIACETIC+ACID'>NTA</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">unda ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1043181 Shewanella sp. HRCR-6])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ufk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ufk OCA], [https://pdbe.org/3ufk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ufk RCSB], [https://www.ebi.ac.uk/pdbsum/3ufk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ufk ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/F8UWD6_9GAMM F8UWD6_9GAMM]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Shewanella sp. hrcr-6]]
+[[Category: Shewanella sp. HRCR_06]]
-[[Category: Clarke, T A]]
+[[Category: Clarke TA]]
-[[Category: Edwards, M J]]
+[[Category: Edwards MJ]]
-[[Category: C-type cytochrome]]
-[[Category: Electron transport]]
-[[Category: Outer membrane]]
-[[Category: Transport protein]]

3ufk: Difference between revisions

Latest revision as of 09:07, 15 November 2023

Crystal structure of UndA complexed with Iron Nitrilotriacetate

Structural highlights

Function

Publication Abstract from PubMed

References

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