7xz3: Difference between revisions

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'''Unreleased structure'''


The entry 7xz3 is ON HOLD  until Paper Publication
==Crystal structure of the Type I-B CRISPR-associated protein, Csh2 from Thermobaculum terrenum==
<StructureSection load='7xz3' size='340' side='right'caption='[[7xz3]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7xz3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobaculum_terrenum Thermobaculum terrenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XZ3 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xz3 OCA], [https://pdbe.org/7xz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xz3 RCSB], [https://www.ebi.ac.uk/pdbsum/7xz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xz3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D1CHV0_THET1 D1CHV0_THET1]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Clustered regularly interspaced short palindromic repeats (CRISPR) in many prokaryotes functions as an adaptive immune system against mobile genetic elements. A heterologous ribonucleoprotein silencing complex composed of CRISPR-associated (Cas) proteins and a CRISPR RNA (crRNA) neutralizes the incoming mobile genetic elements. The type I and III silencing complexes commonly include a protein-helical backbone of several copies of identical subunits, for example, Cas7 in the type I silencing complex. In this study, we structurally characterized type I-B Cas7 (Csh2 from Thermobaculum terrenum; TterCsh2). The revealed crystal structure of TterCsh2 shows a typical glove-like architecture of Cas7, which consists of a palm, a thumb, and a finger domain. Csh2 proteins have 5 conserved sequence motifs that are arranged to form a presumable crRNA-binding site in the TterCsh2 structure. This crRNA binding site of TterCsh2 is structurally and potentially comparable to those observed in helix-forming Cas7 structures in other sub-types. Analysis of the reported Cas7 structures and their sequences suggests that Cas7s can be divided into at least two sub-classes. These data will broaden our understanding on the Cascade complex of CRISPR/Cas systems.


Authors: Seo, P.W., Gu, D.H., Park, S.Y., Kim, J.S.
Structural characterization of the type I-B CRISPR Cas7 from Thermobaculum terrenum.,Seo PW, Gu DH, Kim JW, Kim JH, Park SY, Kim JS Biochim Biophys Acta Proteins Proteom. 2023 May 1;1871(3):140900. doi: , 10.1016/j.bbapap.2023.140900. Epub 2023 Jan 20. PMID:36682394<ref>PMID:36682394</ref>


Description: Crystal structure of the Type I-B CRISPR-associated protein, Csh2 from Thermobaculum terrenum
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Kim, J.S]]
<div class="pdbe-citations 7xz3" style="background-color:#fffaf0;"></div>
[[Category: Park, S.Y]]
== References ==
[[Category: Seo, P.W]]
<references/>
[[Category: Gu, D.H]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermobaculum terrenum]]
[[Category: Gu DH]]
[[Category: Kim JS]]
[[Category: Park SY]]
[[Category: Seo PW]]
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