3wgi

Crystal structure of RSP in complex with beta-NAD+ and operator DNA

spinqualitylabelsall models 3wgi, resolution 3.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wgi is a 8 chain structure with sequence from Thermoanaerobacter ethanolicus JW 200. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.25Å Ligands: NAJ Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D5KM69_THEET Modulates transcription in response to changes in cellular NADH/NAD(+) redox state (By similarity).[HAMAP-Rule:MF_01131][SAAS:SAAS003781_004_005573] Publication Abstract from PubMed The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). Unlike other Rex proteins that regulate aerobic respiration, RSP controls ethanol fermentation in the obligate anaerobe Thermoanaerobacter ethanolicus JW200(T). It is also found in other anaerobic microorganisms. Here we present the crystal structures of apo-RSP, RSP/NADH and RSP/NAD(+)/DNA, which are the first structures of Rex-family members from an obligate anaerobe. RSP functions as a homodimer. It assumes an open conformation when bound to the operator DNA and a closed conformation when not DNA-bound. The DNA binds to the N-terminal winged-helix domain and the dinucleotide, either reduced or oxidized, binds to the C-terminal Rossmann-fold domain. The two distinct orientations of nicotinamide ring, anti in NADH and syn in NAD(+), give rise to two sets of protein-ligand interactions. Consequently, NADH binding makes RSP into a closed conformation, which does not bind to DNA. Both the conserved residues and the DNA specificity of RSP show a number of variations from those of the aerobic Rex, reflecting different structural bases for redox-sensing by the anaerobic and aerobic Rex-family members. Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.,Zheng Y, Ko TP, Sun H, Huang CH, Pei J, Qiu R, Wang AH, Wiegel J, Shao W, Guo RT J Struct Biol. 2014 Dec;188(3):195-204. doi: 10.1016/j.jsb.2014.11.001. Epub 2014, Nov 13. PMID:25463021 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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