3wso: Difference between revisions

Latest revision as of 13:26, 8 November 2023

Crystal structure of the Skp1-FBG3 complex

Structural highlights

3wso is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FBX44_HUMAN Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

Publication Abstract from PubMed

The Skp1-Cul1-F-box protein (SCF) complex catalyzes protein ubiquitination in diverse cellular processes and is one of the best-characterized ubiquitin ligases. F-box proteins determine the substrate specificities of SCF ubiquitin ligases. Among these, Fbs1/FBG1/FBXO2, Fbs2/FBG2/FBXO6, and Fbs3/FBG5/FBXO27 recognize the N-glycans of glycoproteins, whereas FBG3/FBXO44 has no sugar-binding activity, despite the high sequence homology and conservation of the residues necessary for oligosaccharide binding between Fbs1-3 and FBG3. Here we determined the crystal structure of the Skp1-FBG3 complex at a resolution of 2.6 A. The substrate-binding domain of FBG3 is composed of a 10-stranded antiparallel beta-sandwich with three helices. Although the overall structure of FBG3 is similar to that of Fbs1, the residues that form the Fbs1 carbohydrate-binding pocket failed to be superposed with the corresponding residues of FBG3. Structure-based mutational analysis shows that distinct hydrogen bond networks of four FBG3 loops, i.e., beta2-beta3, beta5-beta6, beta7-beta8, and beta9-beta10, prevent the formation of the carbohydrate-binding pocket shown in Fbs1.

The Structural Differences between a Glycoprotein Specific F-Box Protein Fbs1 and Its Homologous Protein FBG3.,Kumanomidou T, Nishio K, Takagi K, Nakagawa T, Suzuki A, Yamane T, Tokunaga F, Iwai K, Murakami A, Yoshida Y, Tanaka K, Mizushima T PLoS One. 2015 Oct 13;10(10):e0140366. doi: 10.1371/journal.pone.0140366., eCollection 2015. PMID:26460611^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumanomidou T, Nishio K, Takagi K, Nakagawa T, Suzuki A, Yamane T, Tokunaga F, Iwai K, Murakami A, Yoshida Y, Tanaka K, Mizushima T. The Structural Differences between a Glycoprotein Specific F-Box Protein Fbs1 and Its Homologous Protein FBG3. PLoS One. 2015 Oct 13;10(10):e0140366. doi: 10.1371/journal.pone.0140366., eCollection 2015. PMID:26460611 doi:https://dx.doi.org/10.1371/journal.pone.0140366

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Kumanomidou T, Nishio K, Takagi K, Nakagawa T, Suzuki A, Yamane T, Tokunaga F, Iwai K, Murakami A, Yoshida Y, Tanaka K, Mizushima T. The Structural Differences between a Glycoprotein Specific F-Box Protein Fbs1 and Its Homologous Protein FBG3. PLoS One. 2015 Oct 13;10(10):e0140366. doi: 10.1371/journal.pone.0140366., eCollection 2015. PMID:26460611 doi:https://dx.doi.org/10.1371/journal.pone.0140366

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='3wso' size='340' side='right'caption='[[3wso]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3wso]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WSO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3wso]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WSO FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2e31|2e31]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FBXO44, FBG3, FBX30, FBX44, FBX6A, FBXO6A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SKP1, EMC19, OCP2, SKP1A, TCEB1L ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wso OCA], [https://pdbe.org/3wso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wso RCSB], [https://www.ebi.ac.uk/pdbsum/3wso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wso ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/FBX44_HUMAN FBX44_HUMAN]] Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. [[https://www.uniprot.org/uniprot/SKP1_HUMAN SKP1_HUMAN]] Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.<ref>PMID:16209941</ref> <ref>PMID:20181953</ref>
+[https://www.uniprot.org/uniprot/FBX44_HUMAN FBX44_HUMAN] Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Iwai, K]]
+[[Category: Iwai K]]
-[[Category: Kumanomidou, T]]
+[[Category: Kumanomidou T]]
-[[Category: Mizushima, T]]
+[[Category: Mizushima T]]
-[[Category: Murakami, A]]
+[[Category: Murakami A]]
-[[Category: Nakagawa, T]]
+[[Category: Nakagawa T]]
-[[Category: Nishio, K]]
+[[Category: Nishio K]]
-[[Category: Suzuki, A]]
+[[Category: Suzuki A]]
-[[Category: Takagi, K]]
+[[Category: Takagi K]]
-[[Category: Tanaka, K]]
+[[Category: Tanaka K]]
-[[Category: Tokunaga, F]]
+[[Category: Tokunaga F]]
-[[Category: Yamane, T]]
+[[Category: Yamane T]]
-[[Category: Yoshida, Y]]
+[[Category: Yoshida Y]]
-[[Category: F-box protein]]
-[[Category: Ligase]]
-[[Category: Scf ubiquitin ligase]]
-[[Category: Skp1]]

3wso: Difference between revisions

Latest revision as of 13:26, 8 November 2023

Crystal structure of the Skp1-FBG3 complex

Structural highlights

Function

Publication Abstract from PubMed

References

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