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==Crystal structure of an inward-facing eukaryotic ABC multitrug transporter G277V/A278V/A279V mutant==
==Crystal structure of an inward-facing eukaryotic ABC multitrug transporter G277V/A278V/A279V mutant==
<StructureSection load='3wmf' size='340' side='right'caption='[[3wmf]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='3wmf' size='340' side='right'caption='[[3wmf]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3wmf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WMF FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WMF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmf OCA], [https://pdbe.org/3wmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wmf RCSB], [https://www.ebi.ac.uk/pdbsum/3wmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmf ProSAT]</span></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wme|3wme]], [[3wmg|3wmg]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmf OCA], [https://pdbe.org/3wmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wmf RCSB], [https://www.ebi.ac.uk/pdbsum/3wmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmf ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
P-glycoprotein is an ATP-binding cassette multidrug transporter that actively transports chemically diverse substrates across the lipid bilayer. The precise molecular mechanism underlying transport is not fully understood. Here, we present crystal structures of a eukaryotic P-glycoprotein homolog, CmABCB1 from Cyanidioschyzon merolae, in two forms: unbound at 2.6-A resolution and bound to a unique allosteric inhibitor at 2.4-A resolution. The inhibitor clamps the transmembrane helices from the outside, fixing the CmABCB1 structure in an inward-open conformation similar to the unbound structure, confirming that an outward-opening motion is required for ATP hydrolysis cycle. These structures, along with site-directed mutagenesis and transporter activity measurements, reveal the detailed architecture of the transporter, including a gate that opens to extracellular side and two gates that open to intramembranous region and the cytosolic side. We propose that the motion of the nucleotide-binding domain drives those gating apparatuses via two short intracellular helices, IH1 and IH2, and two transmembrane helices, TM2 and TM5.
Structural basis for gating mechanisms of a eukaryotic P-glycoprotein homolog.,Kodan A, Yamaguchi T, Nakatsu T, Sakiyama K, Hipolito CJ, Fujioka A, Hirokane R, Ikeguchi K, Watanabe B, Hiratake J, Kimura Y, Suga H, Ueda K, Kato H Proc Natl Acad Sci U S A. 2014 Mar 3. PMID:24591620<ref>PMID:24591620</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wmf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Kato, H]]
[[Category: Kato H]]
[[Category: Kodan, A]]
[[Category: Kodan A]]
[[Category: Nakatsu, T]]
[[Category: Nakatsu T]]
[[Category: Yamaguchi, T]]
[[Category: Yamaguchi T]]
[[Category: Multi drug transporter]]
[[Category: Rec fold]]
[[Category: Transport protein]]

Revision as of 10:34, 31 August 2022

Crystal structure of an inward-facing eukaryotic ABC multitrug transporter G277V/A278V/A279V mutant

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