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==Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin long tail (BTNtail) at 1.5 A resolution==
==Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin long tail (BTNtail) at 1.5 A resolution==
<StructureSection load='3wzo' size='340' side='right'caption='[[3wzo]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='3wzo' size='340' side='right'caption='[[3wzo]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3wzo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WZO FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WZO FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=ZOE:6-({5-[(3AS,4S,5S,6AR)-5-OXIDO-2-OXOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANOYL}AMINO)HEXANOIC+ACID'>ZOE</scene></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wzo OCA], [https://pdbe.org/3wzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wzo RCSB], [https://www.ebi.ac.uk/pdbsum/3wzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wzo ProSAT]</span></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wzn|3wzn]], [[3wzp|3wzp]], [[3wzq|3wzq]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wzo OCA], [https://pdbe.org/3wzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wzo RCSB], [https://www.ebi.ac.uk/pdbsum/3wzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wzo ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
For a multistep pre-targeting method using antibodies, a streptavidin mutant with low immunogenicity, termed low immunogenic streptavidin mutant No. 314 (LISA-314), was produced previously as a drug delivery tool. However, endogenous biotins (BTNs) with high affinity (Kd &lt; 10-10 M) for the binding pocket of LISA-314 prevents access of exogenous BTN-labelled anticancer drugs. In this study, we improve the binding pocket of LISA-314 to abolish its affinity for endogenous BTN species, therefore ensuring that the newly designed LISA-314 binds only artificial BTN analogue. The replacement of three amino acid residues was performed in two steps to develop a mutant termed V212, which selectively binds to 6-(5-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hex anoic acid (iminobiotin long tail, IMNtail). Surface plasmon resonance results showed that V212 has a Kd value of 5.9 x 10-7 M towards IMNtail, but no binding affinity for endogenous BTN species. This V212/IMNtail system will be useful as a novel delivery tool for anticancer therapy.
Structure-based design of a streptavidin mutant specific for an artificial biotin analogue.,Kawato T, Mizohata E, Shimizu Y, Meshizuka T, Yamamoto T, Takasu N, Matsuoka M, Matsumura H, Kodama T, Kanai M, Doi H, Inoue T, Sugiyama A J Biochem. 2015 Feb 2. pii: mvv004. PMID:25645976<ref>PMID:25645976</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wzo" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Avidin 3D structures|Avidin 3D structures]]
*[[Avidin 3D structures|Avidin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Doi, H]]
[[Category: Doi H]]
[[Category: Inoue, T]]
[[Category: Inoue T]]
[[Category: Kanai, M]]
[[Category: Kanai M]]
[[Category: Kawato, T]]
[[Category: Kawato T]]
[[Category: Kodama, T]]
[[Category: Kodama T]]
[[Category: Matsumura, H]]
[[Category: Matsumura H]]
[[Category: Matsuoka, M]]
[[Category: Matsuoka M]]
[[Category: Meshizuka, T]]
[[Category: Meshizuka T]]
[[Category: Mizohata, E]]
[[Category: Mizohata E]]
[[Category: Shimizu, Y]]
[[Category: Shimizu Y]]
[[Category: Sugiyama, A]]
[[Category: Sugiyama A]]
[[Category: Takasu, N]]
[[Category: Takasu N]]
[[Category: Tsumoto, K]]
[[Category: Tsumoto K]]
[[Category: Yamamoto, T]]
[[Category: Yamamoto T]]
[[Category: Beta-barrel]]
[[Category: Biotin binding protein]]

Revision as of 10:39, 31 August 2022

Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin long tail (BTNtail) at 1.5 A resolution

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