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==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==
==Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119==
<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
<StructureSection load='3x0w' size='340' side='right'caption='[[3x0w]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3x0w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X0W FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x0w OCA], [https://pdbe.org/3x0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x0w RCSB], [https://www.ebi.ac.uk/pdbsum/3x0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x0w ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The lysosome is the final destination for degradation of endocytic cargo, plasma membrane constituents, and intracellular components sequestered by macroautophagy. Fusion of endosomes and autophagosomes with the lysosome depends on the GTPase Rab7 and the homotypic fusion and protein sorting (HOPS) complex, but adaptor proteins that link endocytic and autophagy pathways with lysosomes are poorly characterized. Herein, we show that Pleckstrin homology domain containing protein family member 1 (PLEKHM1) directly interacts with HOPS complex and contains a LC3-interacting region (LIR) that mediates its binding to autophagosomal membranes. Depletion of PLEKHM1 blocks lysosomal degradation of endocytic (EGFR) cargo and enhances presentation of MHC class I molecules. Moreover, genetic loss of PLEKHM1 impedes autophagy flux upon mTOR inhibition and PLEKHM1 regulates clearance of protein aggregates in an autophagy- and LIR-dependent manner. PLEKHM1 is thus a multivalent endocytic adaptor involved in the lysosome fusion events controlling selective and nonselective autophagy pathways.
PLEKHM1 Regulates Autophagosome-Lysosome Fusion through HOPS Complex and LC3/GABARAP Proteins.,McEwan DG, Popovic D, Gubas A, Terawaki S, Suzuki H, Stadel D, Coxon FP, Miranda de Stegmann D, Bhogaraju S, Maddi K, Kirchof A, Gatti E, Helfrich MH, Wakatsuki S, Behrends C, Pierre P, Dikic I Mol Cell. 2015 Jan 8;57(1):39-54. doi: 10.1016/j.molcel.2014.11.006. Epub 2014, Dec 11. PMID:25498145<ref>PMID:25498145</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3x0w" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Microtubule-associated protein 3D structures|Microtubule-associated protein 3D structures]]
*[[Microtubule-associated protein 3D structures|Microtubule-associated protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Behrends, C]]
[[Category: Behrends C]]
[[Category: Bhogaraju, S]]
[[Category: Bhogaraju S]]
[[Category: Coxon, F]]
[[Category: Coxon F]]
[[Category: Dikic, I]]
[[Category: Dikic I]]
[[Category: Gatti, E]]
[[Category: Gatti E]]
[[Category: Gubas, A]]
[[Category: Gubas A]]
[[Category: Helfrich, M H]]
[[Category: Helfrich MH]]
[[Category: Kirchhoff, A]]
[[Category: Kirchhoff A]]
[[Category: Maddi, K]]
[[Category: Maddi K]]
[[Category: McEwan, D G]]
[[Category: McEwan DG]]
[[Category: Pierre, P]]
[[Category: Pierre P]]
[[Category: Popovic, D]]
[[Category: Popovic D]]
[[Category: Stadel, D]]
[[Category: Stadel D]]
[[Category: Stegmann, D M]]
[[Category: Stegmann DM]]
[[Category: Suzuki, H]]
[[Category: Suzuki H]]
[[Category: Terawaki, S]]
[[Category: Terawaki S]]
[[Category: Wakatsuki, S]]
[[Category: Wakatsuki S]]
[[Category: Autophagy]]
[[Category: Plekhm1]]
[[Category: Protein binding]]
[[Category: Ubiquitin-like fold]]

Revision as of 10:40, 31 August 2022

Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119

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