1i02: Difference between revisions

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[[Image:1i02.jpg|left|200px]]
{{Seed}}
[[Image:1i02.png|left|200px]]


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{{STRUCTURE_1i02|  PDB=1i02  |  SCENE=  }}  
{{STRUCTURE_1i02|  PDB=1i02  |  SCENE=  }}  


'''NMR STRUCTURE OF CTX A3 AT NEUTRAL PH (20 STRUCTURES)'''
===NMR STRUCTURE OF CTX A3 AT NEUTRAL PH (20 STRUCTURES)===




==Overview==
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Recent studies of cobra P-type cardiotoxins (CTXs) have shown that the water-binding loop (loop II) plays a crucial role in toxin binding to biological membranes and in their cytotoxicity. To understand the role of bound water in the loop, the structure and dynamics of the major P-type CTX from Taiwan cobra, CTX A3, were determined by a comprehensive NMR analysis involving (1)H NOESY/ROESY, (13)C[1)H]NOE/T(1) relaxation, and (17)O triple-quantum filtered NMR. A single water molecule was found to be tightly hydrogen bonded to the NH of Met26 with a correlation time (5-7 ns) approaching the isotropic tumbling time (3.8-4.5 ns) of the CTX A3 molecule. Surprisingly, despite the relatively long residence time (ca. 5 ns to 100 micros), the bound water molecule of CTX A3 is located within a dynamic (order parameter S(2) approximately 0.7) and solvent accessible loop. Comparison among several P-type CTXs suggests that proline residues in the consensus sequence of MxAxPxVPV should play an important role in the formation of the water binding loop. It is proposed that the exchange rate of the bound water may play a role in regulating the lipid binding mode of amphiphilic CTX molecules near membrane surfaces.
The line below this paragraph, {{ABSTRACT_PUBMED_11669614}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_11669614}}


==About this Structure==
==About this Structure==
1I02 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I02 OCA].  
1I02 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I02 OCA].  


==Reference==
==Reference==
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[[Category: Membrane binding protein]]
[[Category: Membrane binding protein]]
[[Category: Three-finger type]]
[[Category: Three-finger type]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:25:04 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:12:35 2008''

Revision as of 07:12, 1 July 2008

File:1i02.png

Template:STRUCTURE 1i02

NMR STRUCTURE OF CTX A3 AT NEUTRAL PH (20 STRUCTURES)

Template:ABSTRACT PUBMED 11669614

About this Structure

1I02 is a Single protein structure of sequence from Naja atra. Full experimental information is available from OCA.

Reference

Dynamic characterization of the water binding loop in the P-type cardiotoxin: implication for the role of the bound water molecule., Sue SC, Jarrell HC, Brisson JR, Wu WG, Biochemistry. 2001 Oct 30;40(43):12782-94. PMID:11669614

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