8gos: Difference between revisions
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==Crystal structure of fluorescent protein RasM== | |||
<StructureSection load='8gos' size='340' side='right'caption='[[8gos]], [[Resolution|resolution]] 1.01Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8gos]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GOS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.01Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gos OCA], [https://pdbe.org/8gos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gos RCSB], [https://www.ebi.ac.uk/pdbsum/8gos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gos ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We perform a beat-frequency-resolved analysis for two-dimensional electronic spectroscopy using a high-speed and stable 2D electronic spectrometer and few-cycle visible laser pulses to disentangle the vibrational coherences in an artificial fluorescent protein. We develop a highly stable ultrashort light source that generates 5.3-fs visible pulses with a pulse energy of 4.7 microJ at a repetition rate of 10 kHz using multi-plate pulse compression and laser filamentation in a gas cell. The above-5.3-fs laser pulses together with a high-speed multichannel detector enable us to measure a series of 2D electronic spectra, which are resolved in terms of beat frequency related to vibrational coherence. We successfully extract the discrete vibrational peaks behind the inhomogeneous broadening in the absorption spectra and the vibrational quantum beats of the excited electronic state behind the strong incoherent population background in the typical 2D electronic spectra. | |||
Beat-frequency-resolved two-dimensional electronic spectroscopy: disentangling vibrational coherences in artificial fluorescent proteins with sub-10-fs visible laser pulses.,Tsubouchi M, Ishii N, Kagotani Y, Shimizu R, Fujita T, Adachi M, Itakura R Opt Express. 2023 Feb 13;31(4):6890-6906. doi: 10.1364/OE.480505. PMID:36823935<ref>PMID:36823935</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8gos" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Discosoma sp]] | |||
[[Category: Large Structures]] | |||
[[Category: Adachi M]] | |||
[[Category: Kagotani Y]] | |||
[[Category: Shimizu R]] | |||
Revision as of 06:57, 27 September 2023
Crystal structure of fluorescent protein RasM
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