1i2m: Difference between revisions

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[[Image:1i2m.png|left|200px]]

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~~'''~~RAN-RCC1-SO4 COMPLEX~~'''~~

===RAN-RCC1-SO4 COMPLEX===

~~==Overview==~~

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~~RCC1 (regulator of chromosome condensation)~~, a beta propeller chromatin-bound protein, is the guanine nucleotide exchange factor (GEF) for the nuclear GTP binding protein Ran. We report here the 1.8 A crystal structure of a Ran*RCC1 complex in the absence of nucleotide, ~~an intermediate in~~ the ~~multistep GEF reaction. In contrast~~ to ~~previous structures,~~ the ~~phosphate binding region of the nucleotide binding site is perturbed only marginally, possibly due to the presence of a polyvalent anion in the P loop~~. ~~Biochemical experiments show that a sulfate ion stabilizes the Ran*RCC1 complex and inhibits dissociation by guanine nucleotides~~. ~~Based on the available structural and biochemical evidence~~, ~~we present a unified scenario for the GEF mechanism~~ where ~~interaction of the P loop lysine with an acidic residue~~ is ~~a crucial element for~~ the ~~overall reaction~~.

The line below this paragraph, {{ABSTRACT_PUBMED_11336674}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 11336674 is the PubMed ID number.

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==About this Structure==

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[[Category: Beta-propeller]]

[[Category: G fold or gtpase fold]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ 2 19:~~29:47~~ 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:19:02 2008''

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-[[Image:1i2m.jpg|left|200px]]
+{{Seed}}
+[[Image:1i2m.png|left|200px]]
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 {{STRUCTURE_1i2m|  PDB=1i2m  |  SCENE=  }}
-'''RAN-RCC1-SO4 COMPLEX'''
+===RAN-RCC1-SO4 COMPLEX===
-==Overview==
+<!--
-RCC1 (regulator of chromosome condensation), a beta propeller chromatin-bound protein, is the guanine nucleotide exchange factor (GEF) for the nuclear GTP binding protein Ran. We report here the 1.8 A crystal structure of a Ran*RCC1 complex in the absence of nucleotide, an intermediate in the multistep GEF reaction. In contrast to previous structures, the phosphate binding region of the nucleotide binding site is perturbed only marginally, possibly due to the presence of a polyvalent anion in the P loop. Biochemical experiments show that a sulfate ion stabilizes the Ran*RCC1 complex and inhibits dissociation by guanine nucleotides. Based on the available structural and biochemical evidence, we present a unified scenario for the GEF mechanism where interaction of the P loop lysine with an acidic residue is a crucial element for the overall reaction.
+The line below this paragraph, {{ABSTRACT_PUBMED_11336674}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 11336674 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_11336674}}
 ==About this Structure==
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 [[Category: Beta-propeller]]
 [[Category: G fold or gtpase fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 19:29:47 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 10:19:02 2008''