1i3x: Difference between revisions

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[[Image:1i3x.gif|left|200px]]
{{Seed}}
[[Image:1i3x.png|left|200px]]


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{{STRUCTURE_1i3x|  PDB=1i3x  |  SCENE=  }}  
{{STRUCTURE_1i3x|  PDB=1i3x  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF THE A LOOP OF 23S RIBOSOMAL RNA'''
===SOLUTION STRUCTURE OF THE A LOOP OF 23S RIBOSOMAL RNA===




==Overview==
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The A loop is an essential RNA component of the ribosome peptidyltransferase center that directly interacts with aminoacyl (A)-site tRNA. The A loop is highly conserved and contains a ubiquitous 2'-O-methyl ribose modification at position U2552. Here, we present the solution structure of a modified and unmodified A-loop RNA to define both the A-loop fold and the structural impact of the U2552 modification. Solution data reveal that the A-loop RNA has a compact structure that includes a noncanonical base pair between C2556 and U2552. NMR evidence is presented that the N3 position of C2556 has a shifted pKa and that protonation at C2556-N3 changes the C-U pair geometry. Our data indicate that U2552 methylation modifies the A-loop fold, in particular the dynamics and position of residues C2556 and U2555. We compare our structural data with the structure of the A loop observed in a recent 50S crystal structure [Ban, N., Nissen, P., Hansen, J., Moore, P. B. &amp; Steitz, T. A. (2000) Science 289, 905--920; Nissen, P., Hansen, J., Ban, N., Moore, P. B. &amp; Steitz, T. A. (2000) Science 289, 920--930]. The solution and crystal structures of the A loop are dramatically different, suggesting that a structural rearrangement of the A loop must occur on docking into the peptidyltransferase center. Possible roles of this docking event, the shifted pKa of C2556 and the U2552 2'-O-methylation in the mechanism of translation, are discussed.
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{{ABSTRACT_PUBMED_11259644}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3X OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3X OCA].  


==Reference==
==Reference==
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[[Category: A loop]]
[[Category: A loop]]
[[Category: A site]]
[[Category: A site]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:32:32 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:22:44 2008''

Revision as of 07:22, 1 July 2008

File:1i3x.png

Template:STRUCTURE 1i3x

SOLUTION STRUCTURE OF THE A LOOP OF 23S RIBOSOMAL RNA

Template:ABSTRACT PUBMED 11259644

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of the A loop of 23S ribosomal RNA., Blanchard SC, Puglisi JD, Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3720-5. Epub 2001 Mar 20. PMID:11259644

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