4rp8: Difference between revisions
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==Bacterial vitamin C transporter UlaA/SgaT in P21 form== | ==Bacterial vitamin C transporter UlaA/SgaT in P21 form== | ||
<StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]]' scene=''> | <StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]], [[Resolution|resolution]] 2.36Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RP8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4rp8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RP8 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [https://pdbe.org/4rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.359Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [https://pdbe.org/4rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/ULAA_ECOLI ULAA_ECOLI] The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active-transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in ascorbate transport. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacteria use vitamin C (L-ascorbic acid) as a carbon source under anaerobic conditions. The phosphoenolpyruvate-dependent phosphotransferase system (PTS), comprising a transporter (UlaA), a IIB-like enzyme (UlaB) and a IIA-like enzyme (UlaC), is required for the anaerobic uptake of vitamin C and its phosphorylation to L-ascorbate 6-phosphate. Here, we present the crystal structures of vitamin C-bound UlaA from Escherichia coli in two conformations at 1.65-A and 2.35-A resolution. UlaA forms a homodimer and exhibits a new fold. Each UlaA protomer consists of 11 transmembrane segments arranged into a 'V-motif' domain and a 'core' domain. The V motifs form the interface between the two protomers, and the core-domain residues coordinate vitamin C. The alternating access of the substrate from the opposite side of the cell membrane may be achieved through rigid-body rotation of the core relative to the V motif. | |||
Crystal structure of a phosphorylation-coupled vitamin C transporter.,Luo P, Yu X, Wang W, Fan S, Li X, Wang J Nat Struct Mol Biol. 2015 Feb 16. doi: 10.1038/nsmb.2975. PMID:25686089<ref>PMID:25686089</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4rp8" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Wang JW]] | [[Category: Wang JW]] | ||
Latest revision as of 15:15, 8 November 2023
Bacterial vitamin C transporter UlaA/SgaT in P21 form
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