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| {{STRUCTURE_1i6o| PDB=1i6o | SCENE= }} | | {{STRUCTURE_1i6o| PDB=1i6o | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF E. COLI BETA CARBONIC ANHYDRASE (ECCA)'''
| | ===CRYSTAL STRUCTURE OF E. COLI BETA CARBONIC ANHYDRASE (ECCA)=== |
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| ==Overview==
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| Carbonic anhydrases fall into three distinct evolutionary and structural classes: alpha, beta, and gamma. The beta-class carbonic anhydrases (beta-CAs) are widely distributed among higher plants, simple eukaryotes, eubacteria, and archaea. We have determined the crystal structure of ECCA, a beta-CA from Escherichia coli, to a resolution of 2.0 A. In agreement with the structure of the beta-CA from the chloroplast of the red alga Porphyridium purpureum, the active-site zinc in ECCA is tetrahedrally coordinated by the side chains of four conserved residues. These results confirm the observation of a unique pattern of zinc ligation in at least some beta-CAS: The absence of a water molecule in the inner coordination sphere is inconsistent with known mechanisms of CA activity. ECCA activity is highly pH-dependent in the physiological range, and its expression in yeast complements an oxygen-sensitive phenotype displayed by a beta-CA-deletion strain. The structural and biochemical characterizations of ECCA presented here and the comparisons with other beta-CA structures suggest that ECCA can adopt two distinct conformations displaying widely divergent catalytic rates.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11316870}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11316870 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11316870}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Ph-dependent activity]] | | [[Category: Ph-dependent activity]] |
| [[Category: Zinc coordination]] | | [[Category: Zinc coordination]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:38:09 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:29:44 2008'' |
