7qba

CryoEM structure of the ABC transporter NosDFY complexed with nitrous oxide reductase NosZ

spinqualitylabelsall models 7qba, resolution 3.78Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7qba is a 7 chain structure with sequence from Pseudomonas stutzeri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ATP, CA, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NOSD_PSEST Required for the assembly of the copper chromophores of nitrous oxide reductase (PubMed:2170125). Could be part of the ABC transporter complex NosDFY (Probable). Publication Abstract from PubMed Emissions of the critical ozone-depleting and greenhouse gas nitrous oxide (N2O) from soils and industrial processes have increased considerably over the last decades(1-3). As the final step of bacterial denitrification, N2O is reduced to chemically inert N2 (refs. (1,4)) in a reaction that is catalysed by the copper-dependent nitrous oxide reductase (N2OR) (ref. (5)). The assembly of its unique [4Cu:2S] active site cluster CuZ requires both the ATP-binding-cassette (ABC) complex NosDFY and the membrane-anchored copper chaperone NosL (refs. (4,6)). Here we report cryo-electron microscopy structures of Pseudomonas stutzeri NosDFY and its complexes with NosL and N2OR, respectively. We find that the periplasmic NosD protein contains a binding site for a Cu(+) ion and interacts specifically with NosL in its nucleotide-free state, whereas its binding to N2OR requires a conformational change that is triggered by ATP binding. Mutually exclusive structures of NosDFY in complex with NosL and with N2OR reveal a sequential metal-trafficking and assembly pathway for a highly complex copper site. Within this pathway, NosDFY acts as a mechanical energy transducer rather than as a transporter. It links ATP hydrolysis in the cytoplasm to a conformational transition of the NosD subunit in the periplasm, which is required for NosDFY to switch its interaction partner so that copper ions are handed over from the chaperone NosL to the enzyme N2OR. Molecular interplay of an assembly machinery for nitrous oxide reductase.,Muller C, Zhang L, Zipfel S, Topitsch A, Lutz M, Eckert J, Prasser B, Chami M, Lu W, Du J, Einsle O Nature. 2022 Aug;608(7923):626-631. doi: 10.1038/s41586-022-05015-2. Epub 2022, Jul 27. PMID:35896743 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA