|
|
| Line 1: |
Line 1: |
| [[Image:1i9f.gif|left|200px]] | | {{Seed}} |
| | [[Image:1i9f.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1i9f| PDB=1i9f | SCENE= }} | | {{STRUCTURE_1i9f| PDB=1i9f | SCENE= }} |
|
| |
|
| '''STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE'''
| | ===STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| INTRODUCTION: The RSG-1.2 peptide was selected for specific binding to the Rev response element RNA, as the natural Rev peptide does. The RSG-1.2 sequence has features incompatible with the helical structure of the bound Rev peptide, indicating that it must bind in a different conformation. RESULTS: The binding of the RSG-1.2 peptide to the Rev response element RNA was characterized using multinuclear, multidimensional NMR. The RSG-1.2 peptide is shown to bind with the N-terminal segment of the peptide along the major groove in an extended conformation and turn preceding a C-terminal helical segment, which crosses the RNA groove in the region widened by the presence of purine-purine base pairs. These features make the details of the bound state rather different than that of the Rev peptide which targets the same RNA sequence binding as a single helix along the groove axis. CONCLUSIONS: These studies further demonstrate the versatility of arginine-rich peptides in recognition of specific RNA elements and the lack of conserved structural features in the bound state.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11358697}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11358697 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_11358697}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9F OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9F OCA]. |
|
| |
|
| ==Reference== | | ==Reference== |
| Line 29: |
Line 33: |
| [[Category: Selected peptide]] | | [[Category: Selected peptide]] |
| [[Category: Solution structure]] | | [[Category: Solution structure]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:44:16 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:37:30 2008'' |
