8e37: Difference between revisions

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'''Unreleased structure'''


The entry 8e37 is ON HOLD  until Paper Publication
==Structure of Campylobacter concisus wild-type SeMet PglC==
<StructureSection load='8e37' size='340' side='right'caption='[[8e37]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8e37]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_concisus_13826 Campylobacter concisus 13826]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E37 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e37 OCA], [https://pdbe.org/8e37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e37 RCSB], [https://www.ebi.ac.uk/pdbsum/8e37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e37 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A7ZET4_CAMC1 A7ZET4_CAMC1]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Complex glycans serve essential functions in all living systems. Many of these intricate and byzantine biomolecules are assembled employing biosynthetic pathways wherein the constituent enzymes are membrane-associated. A signature feature of the stepwise assembly processes is the essentiality of unusual linear long-chain polyprenol phosphate-linked substrates of specific isoprene unit geometry, such as undecaprenol phosphate (UndP) in bacteria. How these enzymes and substrates interact within a lipid bilayer needs further investigation. Here, we focus on a small enzyme, PglC from Campylobacter, structurally characterized for the first time in 2018 as a detergent-solubilized construct. PglC is a monotopic phosphoglycosyl transferase (PGT) that embodies the functional core structure of the entire enzyme superfamily and catalyzes the first membrane-committed step in a glycoprotein assembly pathway. The size of the enzyme is significant as it enables high-level computation and relatively facile, for a membrane protein, experimental analysis. Our ensemble computational and experimental results provided a high-level view of the membrane-embedded PglC/UndP complex. The findings suggested that it is advantageous for the polyprenol phosphate to adopt a conformation in the same leaflet where the monotopic membrane protein resides as opposed to additionally disrupting the opposing leaflet of the bilayer. Further, the analysis showed electrostatic steering acts as a major driving force, contributing to the recognition and binding of both UndP and the soluble nucleotide sugar substrate. Iterative computational and experimental mutagenesis support a specific interaction of undecaprenol phosphate with PGT cationic residues and suggest a role for critical conformational transitions in substrate binding and specificity.


Authors: Vuksanovic, N., Ray, L.C., Imperiali, B., Allen, K.N.
Synergistic computational and experimental studies of a phosphoglycosyl transferase membrane/ligand ensemble.,Majumder A, Vuksanovic N, Ray LC, Bernstein HM, Allen KN, Imperiali B, Straub JE J Biol Chem. 2023 Aug 24:105194. doi: 10.1016/j.jbc.2023.105194. PMID:37633332<ref>PMID:37633332</ref>


Description: Structure of Campylobacter concisus wild-type SeMet PglC
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Vuksanovic, N]]
<div class="pdbe-citations 8e37" style="background-color:#fffaf0;"></div>
[[Category: Imperiali, B]]
== References ==
[[Category: Allen, K.N]]
<references/>
[[Category: Ray, L.C]]
__TOC__
</StructureSection>
[[Category: Campylobacter concisus 13826]]
[[Category: Large Structures]]
[[Category: Allen KN]]
[[Category: Imperiali B]]
[[Category: Ray LC]]
[[Category: Vuksanovic N]]
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