1iin: Difference between revisions

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[[Image:1iin.jpg|left|200px]]
{{Seed}}
[[Image:1iin.png|left|200px]]


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{{STRUCTURE_1iin|  PDB=1iin  |  SCENE=  }}  
{{STRUCTURE_1iin|  PDB=1iin  |  SCENE=  }}  


'''thymidylyltransferase complexed with UDP-glucose'''
===thymidylyltransferase complexed with UDP-glucose===




==Overview==
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Metabolite glycosylation is affected by three classes of enzymes: nucleotidylyltransferases, which activate sugars as nucleotide diphospho-derivatives, intermediate sugar-modifying enzymes and glycosyltransferases, which transfer the final derivatized activated sugars to aglycon substrates. One of the first crystal structures of an enzyme responsible for the first step in this cascade, alpha-D-glucopyranosyl phosphate thymidylyltransferase (Ep) from Salmonella, in complex with product (UDP-Glc) and substrate (dTTP) is reported at 2.0 A and 2.1 A resolution, respectively. These structures, in conjunction with the kinetic characterization of Ep, clarify the catalytic mechanism of this important enzyme class. Structure-based engineering of Ep produced modified enzymes capable of utilizing 'unnatural' sugar phosphates not accepted by wild type Ep. The demonstrated ability to alter nucleotidylyltransferase specificity by design is an integral component of in vitro glycosylation systems developed for the production of diverse glycorandomized libraries.
The line below this paragraph, {{ABSTRACT_PUBMED_11373625}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 11373625 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11373625}}


==About this Structure==
==About this Structure==
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[[Category: Thorson, J S.]]
[[Category: Thorson, J S.]]
[[Category: Transferase]]
[[Category: Transferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:02:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 12:16:54 2008''

Revision as of 09:17, 1 July 2008

File:1iin.png

Template:STRUCTURE 1iin

thymidylyltransferase complexed with UDP-glucose

Template:ABSTRACT PUBMED 11373625

About this Structure

1IIN is a Single protein structure of sequence from Salmonella enterica. Full crystallographic information is available from OCA.

Reference

Structure, mechanism and engineering of a nucleotidylyltransferase as a first step toward glycorandomization., Barton WA, Lesniak J, Biggins JB, Jeffrey PD, Jiang J, Rajashankar KR, Thorson JS, Nikolov DB, Nat Struct Biol. 2001 Jun;8(6):545-51. PMID:11373625

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