1io9: Difference between revisions

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[[Image:1io9.~~jpg~~|left|200px]]

[[Image:1io9.png|left|200px]]

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~~'''~~THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES~~'''~~

===THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES===

~~==Overview==~~

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~~Crystal structures of a thermostable cytochrome P450 (CYP119) and a site-directed mutant~~, ~~(Phe24Leu)~~, ~~from~~ the ~~acidothermophilic archaea Sulfolobus solfataricus were determined~~ at 1.~~5-2~~.~~0 A resolution. We identify important crystallographic waters in the ferric heme pocket, observe protein conformational changes upon inhibitor binding~~, and detect a unique distribution of surface charge not found in other P450s. An analysis of factors contributing to thermostability of CYP119 of these high resolution structures shows an apparent increase in clustering of aromatic residues and optimum stacking. The contribution of aromatic stacking was investigated further with the ~~mutant crystal structure and differential scanning calorimetry~~.

The line below this paragraph, {{ABSTRACT_PUBMED_12237217}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12237217 is the PubMed ID number.

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==About this Structure==

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[[Category: Structural genomic]]

[[Category: Thermophilic]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ ~~2 20~~:12:54 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:40:09 2008''

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-[[Image:1io9.jpg|left|200px]]
+{{Seed}}
+[[Image:1io9.png|left|200px]]
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 {{STRUCTURE_1io9|  PDB=1io9  |  SCENE=  }}
-'''THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES'''
+===THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES===
-==Overview==
+<!--
-Crystal structures of a thermostable cytochrome P450 (CYP119) and a site-directed mutant, (Phe24Leu), from the acidothermophilic archaea Sulfolobus solfataricus were determined at 1.5-2.0 A resolution. We identify important crystallographic waters in the ferric heme pocket, observe protein conformational changes upon inhibitor binding, and detect a unique distribution of surface charge not found in other P450s. An analysis of factors contributing to thermostability of CYP119 of these high resolution structures shows an apparent increase in clustering of aromatic residues and optimum stacking. The contribution of aromatic stacking was investigated further with the mutant crystal structure and differential scanning calorimetry.
+The line below this paragraph, {{ABSTRACT_PUBMED_12237217}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12237217 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12237217}}
 ==About this Structure==
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 [[Category: Structural genomic]]
 [[Category: Thermophilic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:12:54 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 13:40:09 2008''