1isk: Difference between revisions

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[[Image:1isk.gif|left|200px]]
{{Seed}}
[[Image:1isk.png|left|200px]]


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{{STRUCTURE_1isk|  PDB=1isk  |  SCENE=  }}  
{{STRUCTURE_1isk|  PDB=1isk  |  SCENE=  }}  


'''3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES'''
===3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES===




==Overview==
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The three-dimensional structure of the enzyme 3-oxo-delta5-steroid isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by multidimensional heteronuclear magnetic resonance spectroscopy. The two independently folded monomers pack together by means of extensive hydrophobic and electrostatic interactions. Each monomer comprises three alpha helices and a six-strand mixed beta-pleated sheet arranged to form a deep hydrophobic cavity. Catalytically important residues Tyr14 (general acid) and Asp38 (general base) are located near the bottom of the cavity and positioned as expected from mechanistic hypotheses. An unexpected acid group (Asp99) is also located in the active site adjacent to Tyr14, and kinetic and binding studies of the Asp99 to Ala mutant demonstrate that Asp99 contributes to catalysis by stabilizing the intermediate.
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{{ABSTRACT_PUBMED_9103200}}


==About this Structure==
==About this Structure==
1ISK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ISK OCA].  
1ISK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ISK OCA].  


==Reference==
==Reference==
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[[Category: Isomerase]]
[[Category: Isomerase]]
[[Category: Ksi]]
[[Category: Ksi]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:21:52 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:55:45 2008''

Revision as of 10:55, 1 July 2008

File:1isk.png

Template:STRUCTURE 1isk

3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES

Template:ABSTRACT PUBMED 9103200

About this Structure

1ISK is a Single protein structure of sequence from Comamonas testosteroni. Full experimental information is available from OCA.

Reference

Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:9103200

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