8e1w: Difference between revisions
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==Neutron crystal structure of Panus similis AA9A at room temperature== | |||
<StructureSection load='8e1w' size='340' side='right'caption='[[8e1w]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8e1w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Panus_similis Panus similis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8E1W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8E1W FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8e1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8e1w OCA], [https://pdbe.org/8e1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8e1w RCSB], [https://www.ebi.ac.uk/pdbsum/8e1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8e1w ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0S2GKZ1_9APHY A0A0S2GKZ1_9APHY] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes which cleave polysaccharides oxidatively and are important in pathogen biology, carbon cycling and biotechnology. The Lentinus similis family AA9 isoform A (LsAA9_A) has been extensively studied as a model system because its activity towards smaller soluble saccharide substrates has allowed detailed structural characterization of its interaction with a variety of substrates by X-ray crystallography at high resolution. Here, the joint X-ray/neutron room-temperature crystallographic structure of carbohydrate-free LsAA9_A in the copper(II) resting state refined against X-ray and neutron data at 2.1 and 2.8 A resolution, respectively, is presented. The results provide an experimental determination of the protonation states of the copper(II)-coordinating residues and second-shell residues in LsAA9_A, paving the way for future neutron crystallographic studies of LPMO-carbohydrate complexes. | |||
Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature.,Tandrup T, Lo Leggio L, Meilleur F Acta Crystallogr F Struct Biol Commun. 2023 Jan 1;79(Pt 1):1-7. doi: , 10.1107/S2053230X22011335. Epub 2023 Jan 1. PMID:36598350<ref>PMID:36598350</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8e1w" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Panus similis]] | |||
[[Category: Lo Leggio L]] | |||
[[Category: Meilleur F]] | |||
[[Category: Tandrup T]] | |||
Revision as of 07:42, 11 January 2023
Neutron crystal structure of Panus similis AA9A at room temperature
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